| Molecular Weight Distribution of Dissolved Organic Matter in Lake Hongfeng Determined by High Performance Size Exclusion Chromatography （HPSEC） With On-Line UV-Vis Absorbance and Fluorescence Detection |
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| 引用本文： | 岳兰秀,吴丰昌,刘丛强,黎文,王静,梅毅.Molecular Weight Distribution of Dissolved Organic Matter in Lake Hongfeng Determined by High Performance Size Exclusion Chromatography （HPSEC） With On-Line UV-Vis Absorbance and Fluorescence Detection[J].中国地球化学学报,2004,23(3):275-283. |
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| 作者姓名： | 岳兰秀 吴丰昌 刘丛强 黎文 王静 梅毅 |
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| 作者单位： | State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China,Graduate School,Chinese Academy of Sciences,Beijing 100039,China,State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China,State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China,State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China,Graduate School,Chinese Academy of Sciences,Beijing 100039,China,State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China,Graduate School,Chinese Academy of Sciences,Beijing 100039,China,State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China,Graduate School,Chinese Academy of Sciences,Beijing 100039,China |
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| 基金项目： | 中国科学院资助项目,major research projects |
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| 摘 要： | 1IntroductionDissolvedorganicmatter (DOM)isamixtureoforganicsubstancesconsistingmainlyofhighmolecularweightpolymericcompounds (KenandLee,1 974) .Itplaysanimportantroleincarboncyclingandbiogeochemicalmobilizationoftracemetalsandorganiccompoundsintheaquaticenvi ronment (BeffleandLeppard ,1 995) .InrecentyearsmanyresearchershavepaidattentiontothesecomplexesbasedontheMWDofDOM ,sincethefateandtransportoftracechemicalsareinfluencedbyitsmolecularsize (Chiouetal.,1 986) .Themolecularweightorfrac…
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| 关 键 词： | 液化有机物 分子量 平均数 吸收比 荧光性 聚合化合物 湖水生物化学 |
Molecular weight distribution of dissolved organic matter in Lake Hongfeng determined by high performance size exclusion chromatography (HPSEC) with on-line UV-vis absorbance and fluorescence detection |
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| Yue?LanxiuEmail author,Wu?Fengchang,Liu?Congqiang,Li?Wen,Wang?Jing,Mei?Yi.Molecular weight distribution of dissolved organic matter in Lake Hongfeng determined by high performance size exclusion chromatography (HPSEC) with on-line UV-vis absorbance and fluorescence detection[J].Chinese Journal of Geochemistry,2004,23(3):275-283. |
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| Authors: | Email author" target="_blank">Yue?LanxiuEmail author Wu?Fengchang Liu?Congqiang Li?Wen Wang?Jing Mei?Yi |
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| Institution: | (1) State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, 550002 Guiyang, China;(2) Graduate School, Chinese Academy of Sciences, 100039 Beijing, China |
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| Abstract: | The molecular weight distribution (MWD) of dissolved organic matter (DOM) in lake waters from Lake Hongfeng was examined using high performance size exclusion chromatography (HPSEC) with UV-vis absorbance and fluorescence detection. The elution curves obtained by absorbance and fluorescence techniques expressed similar patterns, with the exception of diminishing of large fraction and the peaks behind several seconds in fluorescence chromatograms. According to its molecular weight (MW), DOM in water samples is divided into several fractions: large (>3.5 kDa); medium-large (3.5-2.0 kDa); medium (2.0-1.0 kDa) and small (<1.0 kDa). The average molecular weight was calculated using the elution curve detected by UV-vis absorbance and fluorescence detection techniques. The results showed that the weight-average molecular weight (Mw) and number-average molecular weight (Mn) calculated by UV-vis absorbance techniques range from 1750 to 2050 Dalton and from 1450 to 1850 Dalton, respectively. And the Mw and Mn obtained by fluorescence detection are lower by 50 to 400 Dalton. As a reference, the molecular weight of Fluka humic acid (FHA) is larger than that of water samples by about 200 Dalton. The average molecular weight of DOM for water samples collected in March and July was compared. The results revealed that the molecular weight is lower for water samples obtained in July than that obtained in March, indicating the ambient environment has an influence on the molecular weight, including photo-degradation and biological activity. |
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| Keywords: | high performance size exclusion chromatography dissolved organic matter molecular weight distribution number-average molecular weight weight-average molecular weight UV-vis absorbance fluorescence |
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