| 天山冻土耐冷菌的分离与产低温酶菌株的筛选 |
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| 引用本文： | 白玉,杨大群,王建辉,章高森,徐世健,刘光琇,安黎哲.天山冻土耐冷菌的分离与产低温酶菌株的筛选[J].冰川冻土,2005,27(4):615-618. |
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| 作者姓名： | 白玉 杨大群 王建辉 章高森 徐世健 刘光琇 安黎哲 |
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| 作者单位： | 兰州大学,生命科学学院,甘肃,兰州,730000;中国科学院,寒区旱区环境与工程研究所沙漠与沙漠化重点实验室,甘肃,兰州,730000;兰州大学,生命科学学院,甘肃,兰州,730000;中国科学院,寒区旱区环境与工程研究所沙漠与沙漠化重点实验室,甘肃,兰州,730000 |
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| 基金项目： | 国家自然科学基金;甘肃省农业生物技术研究与应用开发项目;甘肃省自然科学基金;中国科学院"百人计划" |
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| 摘 要： | 在4℃条件下，从天山乌鲁木齐河源区的多年冻土中分离到36株耐冷菌，通过进一步的形态与生理生化特性的研究，发现大多数菌株为革兰氏阴性杆菌，最适生长温度在22℃左右，在37℃下不生长，这些菌株除氨苄青霉素外，对卡那霉素、四环素、和氯霉素都没有抗性，并从中筛选得到11株、3株、11株、8株在4℃下分别具有产低温蛋白酶、低温淀粉酶、低温脂酶、低温纤维素酶特性的耐冷菌，最后，对耐冷菌的环境适应性进行了初步分析。
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| 关 键 词： | 低温酶 耐冷菌 冻土 天山 |
| 文章编号： | 1000-0240(2005)04-0615-04 |
| 修稿时间： | 2004年11月16日 |
Isolation and Screening of Cold-active Enzymes-Producing Psychrotrophic Bacteria from Permafrost in the Tianshan Mountains |
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| BAI Yu,YANG Da-qun,WANG Jian-hui,ZHANG Gao-sen,XU Shi-jian,LIU Guang-xiu,AN Li-Zhe.Isolation and Screening of Cold-active Enzymes-Producing Psychrotrophic Bacteria from Permafrost in the Tianshan Mountains[J].Journal of Glaciology and Geocryology,2005,27(4):615-618. |
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| Authors: | BAI Yu YANG Da-qun WANG Jian-hui ZHANG Gao-sen XU Shi-jian LIU Guang-xiu AN Li-Zhe |
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| Abstract: | 36 cold-adapted strains were isolated under 4 from the permafrost at the headwaters of the Urumchi River, Tianshan Mountains. Studies of morphological, physiological and biochemical properties of these strains found that most of them were gram-negative bacillus, psychrotrophs with optimum growth about 22 and no growth at 37 . The strains are sensitive to Kanamycin,Tetracycline and Chloromycetin, but have some resistance to Ampicillin. Screening of cold-active enzymes-producing bacteria found that 11 strains, 3 strains, 11 strains, 8 strains, respectively, had the capability of producing cold-active protease, amylase, lipase and cellulase at 4 . The adaptive mechanisms of the psychrotrophic bacteria to low temperature environment were primarily analyzed. |
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| Keywords: | cold-active enzymes psychrotrophic bacteria permafrost Tianshan Mountains |
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