| 漠河盆地科学钻探井MK-1地下冷环境中嗜热微生物的多样性分析 |
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| 引用本文： | 李建华,张亚丽,吕杰,杨红兵.漠河盆地科学钻探井MK-1地下冷环境中嗜热微生物的多样性分析[J].冰川冻土,2012,34(3):726-731. |
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| 作者姓名： | 李建华 张亚丽 吕杰 杨红兵 |
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| 作者单位： | 1. 石河子大学化学化工学院,新疆石河子832003／北京化工大学,北京100029
2. 石河子大学化学化工学院,新疆石河子,832003 |
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| 基金项目： | 中国地质调查局地质调查项目“青藏高原冻土带天然气水合物调查评价” |
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| 摘 要： | 在漠河盆地科学钻探井MK-1地下110m冷环境样品中发现大量嗜热微生物存在的证据,针对这些冷环境中的嗜热菌进行了克隆文库分析和分离培养,分别用细菌和古菌16SrDNA引物PCR扩增.结果表明:未发现古菌16SrDNA存在,而嗜热细菌与栖热菌属(Thermus),地芽孢杆菌属(Geoba-cillus)和厌氧芽孢杆菌属(Anoxybacillus)有较近的亲缘关系,通过分离培养分离出3种地芽孢杆菌属(Geobacillus)的细菌,其相似度均为100%.嗜热微生物多存在于地下温暖有机质丰富的生态系统中,了解它们的来源、分布机制及其多样性对于能源和资源的寻找在一定程度上具有指示意义.
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| 关 键 词： | 嗜热菌 漠河盆地 地下冷环境 S rDNA 微生物多样性 |
Thermophilic Microorganisms Diversity in the Underground Cold Environment of Scientific Test Well MK-1 in Mohe Basin |
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| LI Jian-hua,ZHANG Ya-li,Lü Jie,YANG Hong-bing.Thermophilic Microorganisms Diversity in the Underground Cold Environment of Scientific Test Well MK-1 in Mohe Basin[J].Journal of Glaciology and Geocryology,2012,34(3):726-731. |
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| Authors: | LI Jian-hua ZHANG Ya-li Lü Jie YANG Hong-bing |
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| Institution: | 1(1.School of Chemistry and Chemical Engineering,Shihezi University,Shihezi Xinjiang 832003,China; 2.Beijing University of Chemical Technology,Beijing 100029,China) |
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| Abstract: | Some evidence of thermophilic microorganisms has been found in the underground cold environment of Mohe Basin Scientific Test Well MK-1.In this study,the thermophilic microorganisms are studied with phylogenetic analysis and strain isolation.The result with PCR amplification by 16S rDNA gene primer of bacteria and archeal shows that there was no existence of 16S rDNA belonging to archeal and thermophilic has nearly relative relation to Thermus,Geobacillus and Anoxybacillus.Three kinds of genus of Geobacillus,with similarity of 100% each other,were isolated from the sediment samples.Thermophilic microorganisms are common in warm and rich organic ecological system underground.Therefore,understanding their sources,distribution mechanisms and diversity has indicating significance for searching energy and resources. |
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| Keywords: | thermophilic bacteria Mohe basin underground cold environment 16S rDNA microbial diversity |
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