| 祁连山不同海拔低温原油降解菌群的分布特性研究 |
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| 引用本文： | 杨蕊琪,薛林贵,常思静,章高森,陈拓,刘光琇,张勇,高海宁.祁连山不同海拔低温原油降解菌群的分布特性研究[J].冰川冻土,2016(3):785-793. |
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| 作者姓名： | 杨蕊琪 薛林贵 常思静 章高森 陈拓 刘光琇 张勇 高海宁 |
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| 作者单位： | 1. 兰州交通大学化学与生物工程学院 甘肃省极端环境微生物资源与工程重点实验室,甘肃 兰州,730070;2. 甘肃省极端环境微生物资源与工程重点实验室/中国科学院 寒区旱区环境与工程研究所 沙漠与沙漠化重点实验室,甘肃 兰州,730030;3. 河西学院农业与生物技术学院,甘肃 张掖,734000 |
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| 基金项目： | 国家国际科技合作专项项目(2014DFA30330)；甘肃省国际科技合作项目(1304WCGA173)；国家自然科学基金项目(31260090) |
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| 摘 要： | 以祁连山南麓中端野牛沟土壤为研究对象,研究了不同海拔下低温原油降解细菌群落的分布特征.结果显示:研究区域具有原油利用潜力的低温降解菌数量为0.3×105~1.4×105CFU·g-1,随海拔的升高,菌群明显减少;同时通过16S r RNA基因序列分析共发现4个门、7个属、25种细菌,其中假单胞菌属(Pseudomonas)和节杆菌属(Arthrobacter)是优势属,并且位于海拔3 597 m处的菌株原油利用潜力均较高,而变形菌门的不动杆菌属(Acinetobacter)利用原油生长的潜力最高.
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| 关 键 词： | 祁连山 海拔 低温 原油降解菌 |
Elevation-distribution characteristics of the crude oil degrading bacterium in the Qilian Mountains |
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| Abstract: | In this study,the distribution characteristics of low-temperature crude oil degrading bacterial popula-tion in soil from Yeniugou in the south side of the Qilian Mountains was investigated. The results showed that the quantities of degrading bacterium in this region were between 0.3×105~1.4×105 CFU·g-1,which also decreased with altitude. Meanwhile,through 16S rRNA gene sequence analysis,it was detected that there were four phy-lum,seven genera and 25 species;among them,Pseudomonas and Arthrobacter were predominant genera. It was also found that the strains isolated from the altitude of 3 579 m had relatively higher utilization potential and the strains belonging to the genus Acinetobacter had the highest utilization potential for crude-oil. |
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| Keywords: | Qilian Mountains altitude low-temperature crude oil degrading bacterium |
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