| 青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征 |
| |
| 引用本文： | 王凤,王宁练,徐柏青,沈亮,顾政权,刘晓波.青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征[J].冰川冻土,2019,41(4):968-976. |
| |
| 作者姓名： | 王凤 王宁练 徐柏青 沈亮 顾政权 刘晓波 |
| |
| 作者单位： | 中国科学院青藏高原研究所高寒生态学重点实验室,北京100101;中国科学院大学,北京100049;西北大学城市与环境学院,陕西西安710069;中国科学院青藏高原地球科学卓越创新中心,北京100101;中国科学院青藏高原研究所青藏高原环境变化与地表过程重点实验室,北京100101;中国科学院青藏高原地球科学卓越创新中心,北京100101;中国科学院青藏高原研究所青藏高原环境变化与地表过程重点实验室,北京,100101;中国科学院青藏高原研究所高寒生态学重点实验室,北京100101;中国科学院加德满都科教中心,尼泊尔加德满都44618 |
| |
| 基金项目： | 国家自然科学基金项目（41425004）资助 |
| |
| 摘 要： | 对唐古拉哈日钦冰芯表层0.04~3.04 m及深层127.44~130.36 m中可培养细菌进行了对比研究。发现表层和深层可培养细菌数没有表现出显著差异性，表层冰芯中可培养细菌数为0~9.8×103 CFU·mL-1，略高于深层冰芯的0~8.4×103 CFU·mL-1。表层冰芯中微生物总数为103~106 cells·mL-1，深层冰芯中微生物总数为102~103 cells·mL-1。冰芯中可培养细菌属于Firmicutes（厚壁菌门）、Proteobacteria（变形菌门）、Actinobacteria（放线菌门）、Bacteroidetes（拟杆菌门）和Deinococcus-Thermus（异常球菌门） 5大类。但表层和深层可培养细菌属于不同的优势门，表层为Proteobacteria（38%），深层为Firmicutes（42%）。表层和深层优势属均为Bacillus（芽孢杆菌属），占比分别为18%和29%。已有研究表明哈日钦冰芯98.8 m达到了公元1000年的历史记录，因此对比千年尺度上可培养细菌数量及多样性的差异，能够为进一步发掘新基因，丰富微生物多样性，为了解可培养细菌的进化历史奠定基础。
|
| 关 键 词： | 青藏高原 哈日钦冰芯 可培养细菌 表层 深层 |
| 收稿时间： | 2018-07-25 |
| 修稿时间： | 2019-01-18 |
Characteristics of the culturable bacteria in the surface and deep layers of the Tanggula Hariqin ice core,Tibetan Plateau |
| |
| WANG Feng,WANG Ninglian,XU Baiqing,SHEN Liang,GU Zhengquan,LIU Xiaobo.Characteristics of the culturable bacteria in the surface and deep layers of the Tanggula Hariqin ice core,Tibetan Plateau[J].Journal of Glaciology and Geocryology,2019,41(4):968-976. |
| |
| Authors: | WANG Feng WANG Ninglian XU Baiqing SHEN Liang GU Zhengquan LIU Xiaobo |
| |
| Abstract: | A comparative study was carried out on the culturable bacteria groups in the surface layer(0.04-3.04 m depth)and the deep layer(127.44-130.36 m depth)of the Tanggula Hariqin ice core. It was found that there was no significant difference in the number of culturable bacteria between the surface and deep layers. The number of culturable bacteria in the surface was 0-9.8×103 CFU·mL-1,slightly higher than that of deep ice core 0-8.4×103 CFU·mL-1. The total microorganisms cell count in the surface was 103-106 cells·mL-1,while that in the deep layer was 102-103 cells·mL-1. The culturable bacteria in ice core belonged to the five phyla, namely Proteobacteria,Firmicutes,Actinobacteria,Bacteroidetes and Deinococcus-Thermus. However,culturable bacteria at the surface and deep layer belonged to different dominant phylum,the surface is Proteobacteria (38%) and the deep layer is Firmicutes(42%). Nevertheless,Bacillus was the most dominant genus in both layers,with the abundance of 18% and 29% in surface and deep layers,respectively. The previous study shows the 98.8 m depth ice core of Hariqin reached 1000 A. D. historical record. Comparing the differences in the number and diversity of culturable bacteria on the millennium scale,it can further explore new genes,enrich microbial diversity and lay a foundation for understanding the evolution history of culturable bacteria. |
| |
| Keywords: | Tibetan Plateau Hariqin ice core culturable bacteria surface layer deep layer |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《冰川冻土》浏览原始摘要信息 |
| 点击此处可从《冰川冻土》下载免费的PDF全文 |
|
