| 南海东沙深海冷泉区973-5重力柱沉积物古菌多样性 |
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| 引用本文： | 滕田田,苏新,刘浩东,崔鸿鹏,陈芳,程思海,杨胜雄,王宏斌,梁金强,苏丕波.南海东沙深海冷泉区973-5重力柱沉积物古菌多样性[J].现代地质,2020,34(1):104-116. |
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| 作者姓名： | 滕田田 苏新 刘浩东 崔鸿鹏 陈芳 程思海 杨胜雄 王宏斌 梁金强 苏丕波 |
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| 作者单位： | 1.中国地质大学(北京) 生物地质与环境地质国家重点实验室,北京 1000832.中国地质大学(北京) 海洋学院,北京 1000833.广州海洋地质调查局 实验中心,广东 广州 510760 |
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| 基金项目： | 国家重点基础研究发展计划项目(2009CB219502);国家水合物专项项目“南海天然气水合物成矿理论及分布预测研究”专题“神狐钻探区微生物与微生物标志物测试”(GZH201100305-06-02) |
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| 摘 要： | 中国南海东沙一带冷泉发育,但目前国内外对深海冷泉区微生物研究甚少,特别是缺乏利用高通量测序的记录。对东沙深海冷泉区973-5站位(该站位水深约3 000 m)长约935 cm的重力岩心进行了高通量分析。结果显示:该站位微生物细胞丰度为5.3×108~34.0×108个/g,随深度变深而增加,其变化趋势与甲烷含量变化可对比,与粒度、有机碳的变化也具有相关性。测序结果显示,岩心中主要古菌类群是MBGB(39.9%)、C3(15.8%)以及ANME-1(12.0%),随着深度的变化群落组成有所改变。硫酸盐-甲烷界面(SMI,760 cm)上下出现了大量的MBGB和ANME-1类群,pH也不断增加,暗示了这一区域存在不断增强的甲烷厌氧氧化作用。岩心底部出现了一定量的ANME-1和ANME-2类群,暗示除了在SMI附近甲烷氧化和硫酸盐还原反应强烈,其下部可能还有水合物的分解与甲烷的上涌,为ANME类群生存提供了营养物质。与东沙海区其他站位相比,973-5站位的甲烷通量较高,但没有发现产甲烷菌,推测该区沉积物中高浓度的甲烷来源为周边浅部或深部断裂系统运移供给。
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| 关 键 词： | 古菌多样性 16S rRNA 高通量测序 973-5重力柱 冷泉 东沙海域 中国南海 |
| 收稿时间： | 2019-07-30 |
| 修稿时间： | 2019-10-20 |
Archaeal Diversity in Sediments of Core 973-5 from Deep-sea Cold Seep,Dongsha Area in South China Sea |
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| TENG Tiantian,SU Xin,LIU Haodong,CUI Hongpeng,CHEN Fang,CHENG Sihai,YANG Shengxiong,WANG Hongbin,LIANG Jinqiang,SU Pibo.Archaeal Diversity in Sediments of Core 973-5 from Deep-sea Cold Seep,Dongsha Area in South China Sea[J].Geoscience——Journal of Graduate School,China University of Geosciences,2020,34(1):104-116. |
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| Authors: | TENG Tiantian SU Xin LIU Haodong CUI Hongpeng CHEN Fang CHENG Sihai YANG Shengxiong WANG Hongbin LIANG Jinqiang SU Pibo |
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| Institution: | 1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing 100083, China2. School of Ocean Sciences, China University of Geosciences,Beijing 100083, China3. Guangzhou Marine Geological Survey, Guangzhou, Guangdong 510760, China |
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| Abstract: | Cold seeps are well developed in Dongsha area,South China Sea,but researches on microbes in cold seeps have been inadequate,especially when the high-throughput sequencing technique is used.In this paper,the archaeal diversity of gravity core 973-5 was analyzed based on high-throughput sequencing.The core was 935 cm long and was collected from the deepsea cold seep area in Dongsha at a depth of approximately 3,000 m.The cell abundance in the sediments collected by core 973-5 varied from 5.3×10^8 to 34.0×10^8 cells/g,which increased with depth.The change trend of cell abundance was similar to that of the methane content.Additionally,it was related to the grain size and total organic carbon(TOC)content.The results obtained using high-throughput sequencing revealed that the predominant archaeal groups were MBGB,C3 and ANME-1,which accounted for 39.9%,15.8%and 12.0%of the total sequences of archaeal communities,respectively,changing with depth.Large amounts of MBGB and ANME-1 along with the rise of pH in the sediments near the sulfate-methane interface(SMI)at approximately 760 cm might indicate the process of methane anaerobic oxidation.At the bottom of the core,considerable amounts of ANME-1 and ANME-2 were detected.This might demonstrate intensive methane-sulfate redox reaction near SMI as well as the decomposition of gas hydrate and upwelling of methane in the lower part of the core,which are possible nutrients for the ANME group.Compared with other sites in Dongsha area,the higher methane flux was observed in core 973-5,but no methanogens were detected.This suggests that the large quantities of methane might have been transported from the shallow and deep fracture system nearby. |
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| Keywords: | archaeal diversity 16S rRNA high-throughput sequencing core 973-5 cold seep Dongsha area South China Sea |
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