| 硝酸盐氮氧同位素反硝化细菌法测试研究 |
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| 引用本文： | 张翠云,张俊霞,马琳娜,张胜,殷密英,李政红.硝酸盐氮氧同位素反硝化细菌法测试研究[J].地球科学进展,2010,25(4):360-364. |
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| 作者姓名： | 张翠云 张俊霞 马琳娜 张胜 殷密英 李政红 |
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| 作者单位： | 中国地质科学院水文地质环境地质研究所,河北石家庄,050061 |
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| 基金项目： | 国家自然科学基金，地质调查项目下属专题 |
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| 摘 要： | 进入21世纪,硝酸盐氮氧同位素测试技术的显著进展是反硝化细菌法测试技术的建立。反硝化细菌法具有可同时分析浓度低至μg/L的硝酸盐氮氧同位素组成、免去复杂的样品预处理、分析时间大大缩短以及从复杂溶液中只转化NO3-为N2O等优点。实验过程由细菌挑选和培养、样品NO3-转化为N2O、N2O提取纯化、同位素测试以及同位素测试结果校正等几个关键步骤组成。国外研究结果显示该技术不仅可用于海水和淡水中硝酸盐同位素测试,而且还可用于土壤样品,并得到不断的完善和应用;国内建立了这一技术,在包气带和地下水硝酸盐污染研究中取得重要进展。由于该技术诸多优点,建议大力促进这一技术在我国的研究和应用,希望有关部门加大支持力度,进一步完善该技术并推广应用。
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| 关 键 词： | 氮氧同位素 反硝化细茵法 测试技术 |
Advances in the Study of the Denitrifier Method for the Measurement of Isotopic Compositions of Nitrate |
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| ZHANG Cuiyun,ZHANG Junxia,MA Linna,ZHANG Sheng,YIN Miying,LI Zhenghong.Advances in the Study of the Denitrifier Method for the Measurement of Isotopic Compositions of Nitrate[J].Advance in Earth Sciences,2010,25(4):360-364. |
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| Authors: | ZHANG Cuiyun ZHANG Junxia MA Linna ZHANG Sheng YIN Miying LI Zhenghong |
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| Institution: | Institute of Hydrogeology & Environmental Geology, Chinese Academy of Geology Sciences, Shijiazhuang 050061, China |
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| Abstract: | In the 21st century,the significant advances in the measurement of nitrogen and oxygen isotopic compositions of nitrate are the development of analytical techniques of the denitrifier method.The method has many advantages,such as simultaneous analysis of nitrogen and oxygen isotopic compositions of samples with low NO_3~- concentrations down to μg/L,relief from complicated sample pretrcatment,a large reduction in analysis time,only conversion of NO_3~- in samples to N_2 O in complex solutions and so on.The experimental procedure consists of sever- al key steps including bacterium selection and cultivation,conversion of sample NO_3~- to N_2 O.extraction and isotop- ic analysis of N_2 O and correction of isotopic analytical results.Results from foreign researehers showed that the technique Was applicable for the measurement from soil,seawater and freshwater,and was widelv used.This tech-nique Was also developed in and obtained important advances in the contamination study of nitrate in the vadose zone and groundwater.Because of its advanced analytical technique,We suggest great efforts be given to its re-search and application in China.Concerned agencies in charge should give more support to the studv so that the technique can be modified further and applied widely. |
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| Keywords: | The denitrifier method Nitrogen and oxygen isotopes Analytical techniques |
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