| 721铀矿可培养嗜酸性微生物多样性分布 |
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| 引用本文： | 徐蔚云,刘亚洁,徐玲玲,孙丽敏.721铀矿可培养嗜酸性微生物多样性分布[J].华东地质学院学报,2009,32(2):147-151. |
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| 作者姓名： | 徐蔚云 刘亚洁 徐玲玲 孙丽敏 |
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| 作者单位： | 东华理工大学,土木与环境工程学院,江西,抚州,34400
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| 基金项目： | 国家高技术研究发展计划(863计划)，江西省教育厅科技项目，中国国际科技合作项目 |
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| 摘 要： | 嗜酸性微生物是一类生活在极端酸性环境下的微生物，已被广泛用于生物冶金。对721铀矿嗜酸性微生物的群落分布规律进行了初步的研究与探讨。运用双层固体平板培养技术，以琼脂糖为凝固剂，以SJH为底层添加菌，分离样品中的微生物．得到8种嗜酸性微生物，根据不同菌在不同双层固体平板表面的菌落形态特征，并结合16SrRNA基因扩增技术鉴定菌种。占优势的嗜酸性微生物为氧化亚铁硫杆菌（Acidithiobacillus ferrooxidans，简称A．f）.
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| 关 键 词： | 嗜酸性微生物 双层固体平板 菌落形态 |
The Diversity Distribution of Culturable Acidophilic Microorganisms in 721 Uranium Mine |
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| XU Wei-yun,LIU Ya-jie,XU Ling-ling,SUN Li-min.The Diversity Distribution of Culturable Acidophilic Microorganisms in 721 Uranium Mine[J].Journal of East China Geological Institute,2009,32(2):147-151. |
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| Authors: | XU Wei-yun LIU Ya-jie XU Ling-ling SUN Li-min |
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| Institution: | Faculty of Civil and Environmental Engineering;East China Institute of Technology;Fuzhou;JX 344000;China |
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| Abstract: | Acidophilic microorganisms live in the extreme environment,which have been used widely in Biol-eaching.This paper study the distribution of acidophilic microbial community in 721uranium mine basic-ally.Eight acidophilic microorganisms were obtained from a few samples by using the Culture Technique of overlay plates,using agzrose as coagulant,adding an active culture of Acidiphilium SJH to underlayer.Bacter-ia were identified by various characteristics of colonies on different overlay plates,combined with 16... |
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| Keywords: | acidophilic microorganisms overlay plates colony shape |
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