| 西太平洋深海沉积物古菌多样性垂直分布 |
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| 引用本文： | 王峰,萨仁高娃,李铁刚,马学恩,于心科.西太平洋深海沉积物古菌多样性垂直分布[J].地质学报,2010,84(8). |
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| 作者姓名： | 王峰 萨仁高娃 李铁刚 马学恩 于心科 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018
2. 中国科学院海洋研究所海洋地质与环境重点实验室,山东青岛,266071 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目，中国科学院知识创新工程领域前沿项目(编号MGE2008KG06)资助成果 |
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| 摘 要： | 以提取并纯化的西太平洋深海沉积物DNA为模板,利用古菌PCR特异性引物扩增出样品中古菌的16S rDNA片段,构建其克隆文库,建立阳性克隆子RFLP(Restriction Fragment Length Polymor-phism)酶切图谱。据酶切图谱对所获得的120个克隆进行测序,并与数据库中的序列进行比对,从而进行古菌的多样性和系统发育分析。结果表明,沉积物中扩增的16S rDNA古菌序列分别来自泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota),以Marine Benthic Group B(11.8%)、Marine Benthic Group D(13.6%)、Marine Crenarchaeotic Group(68.69%)为主。少量序列为South African Gold Mine Euryarchaeotic Group(1.07%)、Deep-Sea Hydrothermal Vent Euryarchaeotic Group(1.61%)、UIIB(1.25%)、VALIII(1.79%)、Marine Benthic Group E(0.18%)。以上结果表明西太平洋深海沉积物中有丰富多样的古菌群落。
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| 关 键 词： | 西太平洋 沉积物多样性 |
Vertical Distritubion of Archaeal Diversity in Surface Sediment from the Western Pacific Ocean |
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| WANG Feng,Sarengaowa,LI Tiegang,MA Xueen,YU Xinke.Vertical Distritubion of Archaeal Diversity in Surface Sediment from the Western Pacific Ocean[J].Acta Geologica Sinica,2010,84(8). |
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| Authors: | WANG Feng Sarengaowa LI Tiegang MA Xueen YU Xinke |
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| Institution: | WANG Feng1),Sarengaowa2),LI Tiegang2),MA Xueen1),YU Xinke2) 1) College of Animal Science and Veterinary Medicine of Inner Mongolia Agricultural University,Huhhot,010018,2) Institute of Oceanology,Chinese Academy of Sciences,Qingdao,Shandong,266071 |
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| Abstract: | Diversity of archaea was studied in deep-sea sediments from the Western Pacific Ocean by PCR-RFLP and sequence analysis of 16S rDNA and compared with the published sequences in GenBank data-base. Archaeal 16S rDNA sequences were within phylums of Crenarchaeota and Euryarchaeota, respectively. The majority of archaeal phylotypes were Marine Benthic Group B (MBGB), Marine Benthic Group D (MBGD) and Marine Crenarchaeotic Group (MCG).Additional sequences grouped with the South African Gold Mine Euryarchaeotic G... |
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| Keywords: | 16S rDNA |
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