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| 地下水中锰离子氧化细菌的分离与筛选鉴定 | |
| 引用本文： | 路杨,刘相国,杨朔,宁波,张晓英,郝东云.地下水中锰离子氧化细菌的分离与筛选鉴定[J].吉林大学学报(地球科学版),2009,39(6). |
| 作者姓名： | 路杨 刘相国 杨朔 宁波 张晓英 郝东云 |
| 作者单位： | 1. 吉林大学,分子酶学教育部重点实验室,长春,130021 2. 东北师范大学,遗传与细胞研究所,长春,130024 3. 吉林大学,分子酶学教育部重点实验室,长春,130021;吉林省农业科学院,生物技术中心,长春,130033 |
| 摘 要： | 从沈阳张士经济开发区净水厂的成熟生物滤池中,分离筛选得到了3株具有较强Mn~(2+)氧化能力的革兰氏阴性细菌,分别命名为Mn1、Mn2和Mn3.经3个菌株的16S rDNA鉴定及其系统发育学分析表明,菌株Mn1和Mn2为Delftia属,菌株Mn3为Klebsiella属.进一步考察了3株菌对地下水中浓度为2 mg/L的Mn~(2+)氧化及发酵能力.结果表明,3株菌均可在12 h内达到使1 m~3生物滤料成熟时所需的最少生物量;且Mn1、Mn2和Mn3对Mn~(2+)的相对氧化能力分别为96%、94%和85%,为生物滤池法去除地下水中铁锰的快速启动提供了菌种基础. |
| 关 键 词： | Mn~(2+)氧化细菌 筛选与鉴定 系统发育学分析 生物滤池快速启动 地下水 |
Isolation and Identification of Manganese Oxidizing Bacteria in Groundwater |
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| LU Yang,LIU Xiang-guo,YANG Shuo,NING Bo,ZHANG Xiao-ying,HAO Dong-yun.Isolation and Identification of Manganese Oxidizing Bacteria in Groundwater[J].Journal of Jilin Unviersity:Earth Science Edition,2009,39(6). | |
| Authors: | LU Yang LIU Xiang-guo YANG Shuo NING Bo ZHANG Xiao-ying HAO Dong-yun |
| Institution: | LU Yang1,LIU Xiang-guo1,YANG Shuo1,NING Bo1,ZHANG Xiao-ying2,HAO Dong-yun1,31.Key Laboratory for Molecular Enzymology , Engineering of the Ministry of Education,Jilin University,Changchun 130021,China2.Institute of Genentics , Cytology,Northeast Normal University,Changchun 130024,China3.Biotechnology Research Centre,Jilin Academy of Agricultural Sciences(JAAS),Changchun 130033,China |
| Abstract: | Three strains of gram-negative bacteria,named Mn1,Mn2 and Mn3,which were capable of effective Mn2+ oxidization were identified in the water of water-supply plant in Zhangshi economic development zone,Shenyang,PRC.Phylogenetic analysis showed that strain Mn1 and strain Mn2 may belong to the family of Delftia,while strain Mn3 was in the family of Klebsiella.The capability of Mn2+ oxidation and fermentation characteristics of these three bacteria were measured using groundwater containing 2 mg/L Mn2+.The resul... |
| Keywords: | manganese-oxidizing bacteria screening and identification phylogenetic analysis rapid start-up of biofilter groundwater |
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