| 南极长城站空气微生物的分子生态学分析 |
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| 引用本文： | 夏晓敏,汪建君,陈立奇,颜金培,张锐.南极长城站空气微生物的分子生态学分析[J].极地研究,2014,26(2):186-192. |
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| 作者姓名： | 夏晓敏 汪建君 陈立奇 颜金培 张锐 |
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| 作者单位： | 1.厦门大学近海海洋环境科学国家重点实验室，福建 厦门 361005;
;2.厦门大学海洋微型生物与地球圈层研究所，福建 厦门 361005;
;3.国家海洋局第三海洋研究所，海洋-大气化学与全球变化重点实验室，福建 厦门 361005 |
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| 基金项目： | 国家自然科学基金(41106168);国家海洋局海洋-大气化学与全球变化重点实验室开放基金(GCMAC1003);厦门大学基础创新科研基金(中央高校基本科研业务费专项资金2011121012)资助 |
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| 摘 要： | 微生物是气溶胶的重要组成部分。本研究通过收集南极长城站附近气溶胶,构建气溶胶中空气微生物16S rRNA基因文库,发现南极空气微生物具有很高的多样性,93个克隆子包含了53个OTU(Operational Taxonomic Unit),Shannon指数为3.58,Simpson指数为0.04。南极长城站空气微生物的主要细菌类群为Actinobacteria、Cyanobacteria/Chloroplasts、Bacteroidetes、Firmicutes、Planctomycetes、Proteobacteria及Verrucomicrobia等7个门,Proteobacteria门的细菌是优势类群,占长城站空气微生物细菌克隆文库的65.7%。克隆文库中海源微生物序列及陆源微生物序列各占30.1%,说明长城站附近具有较强的海陆大气交换。我们发现有15个克隆子序列与人类活动相关,占总克隆子数的16.1%,说明人类活动已经影响到了长城站的空气微生物群落结构。
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| 关 键 词： | 南极空气微生物 微生物群落结构 16S rRNA基因文库 |
| 收稿时间： | 2013-02-04 |
BIODIVERSITY OF AIRBORNE BACTERIAAT THE GREAT WALL STATION,ANTARCTICA |
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| Xia Xiaomin,Wang Jianjun,Chen liqi,Yan Jinpei,Zhang Rui.BIODIVERSITY OF AIRBORNE BACTERIAAT THE GREAT WALL STATION,ANTARCTICA[J].Chinese Journal of Polar Research,2014,26(2):186-192. |
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| Authors: | Xia Xiaomin Wang Jianjun Chen liqi Yan Jinpei Zhang Rui |
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| Institution: | 1.State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;2.Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen 361005, China;3.Key Laboratory of Global Change and Marine-Atmospheric Chemistry, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: | Bacteria are an important component of the aerosol, which is closely related with the human beings. This study analyzed the community structure of air-borne bacteria at The Great Wall Station, Antarctica, based on 16S rRNA gene clone library analysis. High diversity of air-borne bacteria was observed in the aerosol samples collected at The Great Wall Station. Ninety three sequences from the clone library can be classified into 53 OTUs (operation taxonomic unit) based on 97% sequence similarity. Shannon and Simpson index of the clone library are 3.58 and 0.04, respectively. Phylogenetic analysis showed the sequences can be affiliated into seven phylum: Actinobacteria、Cyanobacteria/Chloroplast、Bacteroidetes、Firmicutes、Planctomycetes、Proteobacteria and Verrucomicrobia. Proteobacterial sequences accounted for 65.6% of the clone library. There were same amount of sequences (30.1%) seemed to be derived from marine and terrestrial environments, suggesting a strong atmosphere exchange of the land and sea near The Great Wall Station. In addition, there were 15 sequences related with human activities, which indicated that human activities had already affected the airborne microbial community structure of The Great Wall Station. |
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| Keywords: | Antarctica aerosol bacteria community structure 16S rRNA gene clone library |
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