| 南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测 |
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| 引用本文： | 王飞,丛柏林,张朝晖,杨黄浩.南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测[J].极地研究,2020,32(1):68-81. |
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| 作者姓名： | 王飞 丛柏林 张朝晖 杨黄浩 |
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| 作者单位： | 福州大学,福建福州350108;自然资源部第一海洋研究所,山东青岛266061 |
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| 基金项目： | “十三五”国家重点研发计划(2018YFC1406701)资助 |
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| 摘 要： | 研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。
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| 关 键 词： | 南极罗斯海 可培养菌株 胞外酶 |
| 收稿时间： | 2019-09-24 |
Isolation of culturable strains from Antarctic Ross Sea sediments and study of extracellular hydrolase activity |
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| Wang Fei,Cong Bailin,Zhang Zhaohui,Yang Huanghao.Isolation of culturable strains from Antarctic Ross Sea sediments and study of extracellular hydrolase activity[J].Chinese Journal of Polar Research,2020,32(1):68-81. |
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| Authors: | Wang Fei Cong Bailin Zhang Zhaohui Yang Huanghao |
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| Institution: | 1.Fuzhou University, Fu Zhou 350108, China;
2.First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China |
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| Abstract: | To evaluate the potential of Antarctic microbial resources for exploitation, we isolated culturable bacteria from sediments collected from the Ross Sea region in Antarctica and examined their diversity and extracellular enzyme activities. Pure cultured bacteria and fungi were isolated using Zobell 2216E medium and potato dextrose agar(PDA). Diversity and phylogeny of culturable microorganisms were analyzed using 16S rDNA and 18S/ITS sequencing. We found 79 strains of bacteria and 54 strains of fungi, of which Psychrobacter and Cladosporium were dominant. Phenotypic similarity with model strain was low (less than 97%) in two bacterial strains, indicating the potential discovery of new species. Results from API20NE tests show that most strains have extracellular hydrolase activity. This study provides new information on culturable microbial resources in Antarctica and lays the foundation for exploitation of Antarctic microbial resources. |
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| Keywords: | Ross Sea culturable strains ectoenzyme |
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