| 一株南极细菌（Pseudoalteromonas sp.）对模拟海水养殖水体的净化作用 |
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| 引用本文： | 迟明磊,阚光锋,史翠娟,路来风,林学政,刘胜浩.一株南极细菌（Pseudoalteromonas sp.）对模拟海水养殖水体的净化作用[J].极地研究,2010,22(3):313-320. |
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| 作者姓名： | 迟明磊 阚光锋 史翠娟 路来风 林学政 刘胜浩 |
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| 作者单位： | 1. ;
2. 哈尔滨工业大学（威海）;
3. 国家海洋局第一海洋研究所 |
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| 基金项目： | 山东省自然科学基金;国家海洋局海洋生物活性物质重点实验室开放基金;威海市科技计划项目 |
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| 摘 要： | 试验选用一株高产低温蛋白酶的南极细菌菌株Pseudoalteromonas sp. AN64，从溶解氧、化学需氧量、氨氮、亚硝态氮、pH值5个方面研究了南极细菌对养殖水体的影响。结果显示，随着饲料中蛋白质的散失，水体中蛋白质的含量在第7天达到高峰，溶解氧下降，化学需氧量持续上升，在第11天达最大值9.88 mg/L，并且维持在较高的水平。缺氧条件下，有害物质氨氮和亚硝态氮含量逐渐增加，在第9天和第11天分别达最大值0.65 mg/L和0.352 mg/L，而pH值持续下降，在第9天达最低值5.95。当水体中加入高产蛋白酶的南极菌株AN64后，蛋白质含量在第5天即达高峰，溶解氧无明显变化，化学需氧量在第7天达最高峰，比对照组提前4天，但含量仅约为后者的一半。同时，氨氮和亚硝态氮含量远低于对照组，分别仅为对照的47.7 %和26.5 %，而pH值有小幅下降，维持在6.85到7.52之间。由此可以看出，南极菌株产生的低温蛋白酶可以有效分解海水中的蛋白质，降低水体中的氨氮和亚硝态氮等有害物质，维持pH值的稳定性，为健康养殖提供了有效途径。
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| 关 键 词： | 水质净化 |
| 收稿时间： | 2010-03-17 |
| 修稿时间： | 2010-06-26 |
APPLICATION OF AN ANTARCTIC BACTERIUM STRAIN FOR WATER PURIFICATION IN SIMULATED AQUACULTURE |
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| Chi Minglei,Kan Guangfeng,Shi Cuijuan,Lu Laifeng,Lin Xuezheng,Liu Shenghao.APPLICATION OF AN ANTARCTIC BACTERIUM STRAIN FOR WATER PURIFICATION IN SIMULATED AQUACULTURE[J].Chinese Journal of Polar Research,2010,22(3):313-320. |
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| Authors: | Chi Minglei Kan Guangfeng Shi Cuijuan Lu Laifeng Lin Xuezheng Liu Shenghao |
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| Abstract: | Antarctic bacterium Pseudoalteromonas sp. AN64 producing cold-active protease was used for water purification in this experiment. Effect of strain AN64 on aquaculture seawater was studied from different aspects, including dissoluble oxygen (DO), chemical oxygen demand (COD), ammonia and nitrite nitrogen and pH values. In untreated water, soluble protein content increased and reached the maximum on the 7th day with dissolution of protein. Developing with the time, DO content decreased continuously, while COD content increased and reached its maximum of 9.88 mg/L on the 11th day and sustained at a high level. In the absence of oxygen, some harmful substances, such as ammonia and nitrite nitrogen, increased and came to their maximum on the 9th and 11th day, respectively. The pH value decreased to the minimum of 5.95 on the 9th day. After treatment with Antarctic bacterium producing cold-active protease, protein content in sea water reached the maximum on the 5th day, while DO value kept fairly stable. The maximum content of COD occurred on the 7th day, earlier 4 days than the control did. At the same time, the contents of ammonia and nitrite nitrogen were only 47.7 % and 26.5 % of the control group, respectively. The change of pH was slightly and maintained between 6.85 and 7.52. It indicates that the cold-active protease produced by Antarctic bacterium can hydrolyze effectively protein in water. The low-content protein decreased the production of ammonia and nitrite nitrogen and kept the stability of pH, which provided an effective way for healthy marine aquaculture. |
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| Keywords: | water purification |
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