| 北极海冰细菌产胞外酶及主要环境因子的初步研究 |
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| 引用本文： | 曾胤新,俞勇,涂雪梅,陈波,何剑锋.北极海冰细菌产胞外酶及主要环境因子的初步研究[J].极地研究,2003,15(4):303-309. |
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| 作者姓名： | 曾胤新 俞勇 涂雪梅 陈波 何剑锋 |
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| 作者单位： | 1. 中国极地研究中心,上海,200129
2. 上海水产大学生物科学系,上海,200090 |
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| 基金项目： | 国家自然科学基金面上项目 ( 3 0 2 0 0 0 0 1，40 0 0 60 1 0 )，科技部社会公益研究专项资金项目( 2 0 0 1DIA5 0 0 40— 6)，国家海洋局科学基金项目资助 |
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| 摘 要： | 覆盖在北极海洋上的广阔海冰为其内部生存的微生物群落提供了一个独特的生境。研究表明 ,大量海冰细菌能够分泌产生胞外酶 ,其中产蛋白或脂质水解酶细菌的比例远高于产多糖水解酶的细菌。温度、盐度是直接影响海冰细菌生存与活力的 2个主要环境因子。 76%的产酪蛋白酶海冰细菌为低温菌 ,菌株只能在 <35°C条件下生长 ;而 98%的酪蛋白酶最适作用温度≥ 35°C ,其中 62 %的酶最适作用温度≥ 45°C。几乎所有的产酪蛋白酶海冰细菌都耐盐或嗜盐
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| 关 键 词： | 北极 海冰细菌 酶 环境因子 |
| 修稿时间： | 2003年9月1日 |
A PRIMARY STUDY ON EXTRACELLULAR ENZYME PRODUCTION OF ARCTIC SEA-ICE BACTERIA AND THE EFFECT OF LEADING ENVIRONMENT FACTORS |
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| Zeng Yinxin ,Yu Yong ,Tu Xuemei ,Chen Bo and He Jianfeng.A PRIMARY STUDY ON EXTRACELLULAR ENZYME PRODUCTION OF ARCTIC SEA-ICE BACTERIA AND THE EFFECT OF LEADING ENVIRONMENT FACTORS[J].Chinese Journal of Polar Research,2003,15(4):303-309. |
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| Authors: | Zeng Yinxin Yu Yong Tu Xuemei Chen Bo and He Jianfeng |
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| Institution: | Zeng Yinxin 1,Yu Yong 1,Tu Xuemei 2,Chen Bo 1 and He Jianfeng 1 |
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| Abstract: | Sea ice covering Arctic sea provides a unique habitat with lower temperature and higher salinity, containing a complex microbial community. Numerous sea ice bacteria could produce extracellular enzymes, and the property of enzymes hydrolyzing proteins or lipids was much higher than that hydrolyzing polysaccharids. Temperature and salinity were two leading environment factors directly affecting the survival and activity of sea ice bacteria. This paper showed, with a high growth temperature limit at 35 °C , 76% of sea ice bacteria producing protease was cold adapted. However, 98% of those proteases had an optimum temperature higher than 35°C , and 62% of them even higher than 45 °C . Nearly all sea ice bacteria producing protease were halophilous or salt adapted. |
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| Keywords: | Arctic sea ice bacteria enzyme environment factor |
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