Diversity and genetic differentiation in Artemia species and populations detected by AFLP markers |
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| Authors: | Yi Sun Wen-Qin Song Yi-Cheng Zhong Run-Sheng Zhang Theodore J Abatzopoulos Rui-Yang Chen |
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| Institution: | (1) Department of Biology, Nankai University, 300071 Tianjin, PR China;(2) Department of Genetics, Development and Molecular Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 540 06 Thessaloniki, Greece |
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| Abstract: | Amplified Fragment Length Polymorphism (AFLP) markers were successfully employed to analyze 15Artemia species and strains for genetic diversity. AFLP markers are extremely sensitive to even a small sequence variation. They
are stable and more polymorphic than RAPD. Twelve pairs of primer combinations were used to detect AFLP bands, of which 384
were polymorphic, and DNA fingerprintings were obtained by using silver staining. The polymorphism analysis leads us to the
following conclusions: 1.Artemia tibetiana seems to differentiate fromA. sinica. 2. The parthenogenetic populations from inland salt lakes could follow an evolutionary path that is different from that
of the coastal parthenogenetic populations. |
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| Keywords: | AFLP Artemia species |
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