In situ stress is not only a vital indicator for selecting explorative regions of coalbed methane (CBM), but also a pivotal factor affecting CBM production. The present study explored whether in situ stress affected the development potential of CBM in western Guizhou, China. To this end, we collected injection/falloff well test data and gas content data from 70 coal seams in 28 wells. The study found that from top to bottom, strike slip fault stress fields (<?500 m), normal fault stress fields (500–1000 m) and strike slip fault stress fields (>?1000 m) were successively developed in western Guizhou. The distribution features of vertical permeability in western Guizhou are consistent with the stress fields' transformation location. The coal permeability in the western part in Guizhou presents a tendency of increase followed by decrease as a result of increased burial depth. The vertical development characteristics of coal seam gas content are controlled mainly by reservoir pressure, and the relationship between reservoir pressure and buried depth shows a linear increase. The CBM in western Guizhou is divided vertically into three development potential regions dependent on the characteristics of burial depth, permeability and gas content of coal seams. The most favorable vertical development potential region in western Guizhou is 500–1000 m. This region exhibits high gas content, high permeability and moderate burial depth, which are favorable for the production of CBM. These research results can provide basis for geological selection and engineering implementation of CBM in western Guizhou.