| 混合盐碱胁迫对紫花苜蓿苗期氮磷吸收及生理特性的影响 |
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| 引用本文： | 郝凤,刘晓静,张晓磊,齐敏兴.混合盐碱胁迫对紫花苜蓿苗期氮磷吸收及生理特性的影响[J].中国沙漠,2015,35(5):1268-1274. |
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| 作者姓名： | 郝凤 刘晓静 张晓磊 齐敏兴 |
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| 作者单位： | 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/中美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070 |
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| 基金项目： | 国家自然科学基金项目(31460622);公益性行业(农业)科研专项(201403048-8) |
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| 摘 要： | 以“甘农3号”紫花苜蓿为试验材料,采用营养液沙培试验,用两种中性盐(NaCl和Na2SO4)及两种碱性盐(NaHCO3和Na2CO3), 配成5种混合盐A(NaCl:Na2SO4=1:1)、B(NaCl:NaHCO3=1:1)、C(NaCl:Na2SO4:NaHCO3:Na2CO3=1:1:1:1)、D(Na2SO4:Na2CO3=1:1)、E(NaHCO3:Na2CO3=1:1),设4个盐浓度(25、50、100、150 mmol·L-1)模拟盐胁迫,研究混合盐碱胁迫对紫花苜蓿苗期氮、磷吸收及生理特性的影响。结果表明:随着盐浓度增加,苜蓿根瘤固氮酶活性和植株全氮含量下降,全磷含量无明显变化,而脯氨酸(Pro)、丙二醛(MDA)、可溶性糖含量和超氧化物歧化酶(SOD)活性均升高,同一盐浓度水平下各处理间其含量的升高顺序为E>D>C>B>A,在高胁迫强度下(150 mmol·L-1) 除全磷含量外,各项指标增减均达显著水平。混合盐碱胁迫能够对紫花苜蓿根瘤固氮酶组分结构造成不可逆的破坏,使苜蓿自身固氮能力的下降,最终导致植株全氮含量下降。随着盐浓度的上升,植株可以通过提高其体内脯氨酸和可溶性糖的含量来维持细胞水势,减少细胞渗透胁迫对植物的伤害。
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| 关 键 词： | 紫花苜蓿 混合盐碱胁迫 固氮酶活性 生理指标 |
| 收稿时间： | 2014-11-04 |
| 修稿时间： | 2014-12-28 |
Effect of Complex Saline-alkali Stress on Nitrogen and Phosphorus Absorption and Physiological Traits of Alfalfa Seedlings |
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| Hao Feng,Liu Xiaojing,Zhang Xiaolei,Qi Minxing.Effect of Complex Saline-alkali Stress on Nitrogen and Phosphorus Absorption and Physiological Traits of Alfalfa Seedlings[J].Journal of Desert Research,2015,35(5):1268-1274. |
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| Authors: | Hao Feng Liu Xiaojing Zhang Xiaolei Qi Minxing |
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| Institution: | College of Grassland Science/Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S.Centers for Grazing land Ecosystem Sustainability, Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | The effect of complex saline-alkali stress on nitrogen and phosphorus absorption and physiological characteristics of alfalfa seedlings were studied with Alfalfa (Medicago sativa, Gannong No.3). Seedlings were stressed with different concentrations of neural salts (NaCl and Na2SO4) and alkaline salts (NaHCO3 and Na2CO3), altogether 5 combinations: A(NaCl:Na2SO4=1:1), B(NaCl:NaHCO3=1:1), C(NaCl:Na2SO4:NaHCO3:Na2CO3=1:1:1:1), D(Na2SO4:Na2CO3=1:1)、E(NaHCO3:Na2CO3=1:1), and 4 concentrations (25, 50, 100 and 150 mmol·L-1) in a sand culture experiment. Results show that with the increase of salt concentration, nodule nitrogenase activity and the content of total nitrogen has dropped, and the content of total phosphorus has no obvious change, while proline (Pro), malondialdehyde (MDA), soluble sugar and superoxide dismutase (SOD) content increased. At the same level of the salt concentration, the content between the groups of higher order: E>D>C>B>A.The change ranges of tested indices have reached significant level under the high salt concentration(150 mmol·-1), in addition to the total phosphorus content.The results suggested that the structure components of alfalfa was damaged under the hybrid salinity-alkalinity stress,reduced nitrogen fixation ability and decreased nitrogen content. Along with the rising of salt concentrations, plant can improve the content of proline and soluble sugar in the body to maintain cell water potential, reduce the injury to the plant cell osmotic stress. |
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| Keywords: | alfalfa (Medicago sativa) salt and alkali mixed stress nitrogenase activity physiological index |
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