| 不同有机溶剂萃取生物结皮中叶绿素a效率的比较研究 |
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| 引用本文： | 兰书斌,刘永定,胡春香.不同有机溶剂萃取生物结皮中叶绿素a效率的比较研究[J].中国沙漠,2009,29(3):524-528. |
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| 作者姓名： | 兰书斌 刘永定 胡春香 |
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| 作者单位： | 1.中国科学院 水生生物研究所, 湖北 武汉 430072; 2.中国科学院研究生院, 北京 100049 |
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| 基金项目： | 国家自然科学基金，中国科学院知识创新领域前沿项目，武汉市科技局与内蒙古发改委重大科技产业化专项基金 |
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| 摘 要： | 参照水体中浮游植物叶绿素a的萃取测定方法，运用丙酮、乙醇、二甲基甲酰胺（DMF）、二甲基亚砜（DMSO）4种有机溶剂对不同土质不同发育阶段自然和人工生物结皮中叶绿素a的萃取效率进行了比较研究。结果发现，不同有机溶剂从生物结皮中萃取叶绿素a的效率存在极显著差异（P<0.001），而乙醇法的离散度最低；取样量对萃取效率总体上没有显著影响（P>0.05），但在具体的测定中与萃取效率之间存在负相关。4种方法中，DMSO法对藻类占优势的结皮萃取率最高，DMF法对自然结皮的萃取率仅次于DMSO法，在人工结皮中最低；乙醇法和丙酮法的萃取效率普遍较低，而且与结皮属性之间没有明显规律。
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| 关 键 词： | 有机溶剂 萃取 生物结皮 叶绿素a |
| 收稿时间： | 2008-01-02; |
| 修稿时间： | 2008-04-10 |
Comparison of Contents of Chlorophyll-a Extracted by Different Organic Solvents in Biological Crusts |
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| LAN Shu-bin,LIU Yong-ding,HU Chun-xiang.Comparison of Contents of Chlorophyll-a Extracted by Different Organic Solvents in Biological Crusts[J].Journal of Desert Research,2009,29(3):524-528. |
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| Authors: | LAN Shu-bin LIU Yong-ding HU Chun-xiang |
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| Institution: | 1.Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, China; 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Based on the extraction and measurement of phytoplanktonic Chl-a in water, this study compared contents of Chl-a extracted by acetone, ethanol, N,N-Dimethylformamide (DMF) and Dimethyl Sulphoxide (DMSO) in natural and man-made biological crusts that were in different soil texture and stages of development. The results showed that there was significant statistical difference in content of Chl-a extracted by different organic solvents (P<0.001), while scatteration was the least in ethanol method. Although the sample content hadnt prominent effects on content of Chl-a extraction as a whole (P>0.05), there was a negative correlation between content of Chl-a extraction and sample content. Comparisons displayed that in the algae dominative crusts, content of Chl-a extraction was the highest in DMSO method, while lower in DMF than DMSO method in natural crusts, but the lowest in the man-made crusts. Generally, the extraction rate was lower in acetone and ethanol method, which also hadnt evident relation to the crusts attributes. |
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| Keywords: | organic solvent extraction biological crust Chl-a |
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