Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization |
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| Authors: | Baoyu Zhang Guofu Chen Guangce Wang Douding Lu |
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| Institution: | (1) Faculty of Marine Technology, Chonnam National University, Yeosu, Jeollanam-do, 550-749, Republic of Korea;(2) Department of Ocean Engineering, Chonnam National University, Yeosu, Jeollanam-do, 550-749, Republic of Korea |
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| Abstract: | A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant
environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently
required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2
from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including
5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence
microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA
of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding
was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising
tool for detecting target HAB species in natural environments. |
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