Use of tetracycline hydrochloride and alizarin complexone for immersion marking black rockfish Sebastes schlegelii |
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| Authors: | Hongjian Lü Xiumei Zhang Mei Fu Dan Xi Tianxiang Gao |
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| Institution: | 1. College of Fisheries, Ocean University of China, Qingdao, 266003, China
2. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education) College of Animal Science and Technology, Southwest University, Chongqing, 400715, China
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| Abstract: | We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles (30–40 mm total length) were immersed in a range of tetracycline hydrochloride (TC) solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone (ALC) solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water (salinity of 30) for 24 h, respectively. Otoliths (sagittae, asteriscus), scales, fin rays (dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines (dorsal, ventral, and anal fin spines) were sampled and used to detect fluorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of 200–400 mg/L, 250–400 mg/L, and 250–400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations (≥350 mg/L TC, ≥250 mg/L ALC for scales and ≥350 mg/L TC, ≥300 mg/L ALC for fin rays). The best mark quality (i.e., acceptable marks were observed in all sampled structures after immersion marking) were obtained following immersion in TC at between 350–500 mg/L, and ALC between 300–400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking (P > 0.05). |
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| Keywords: | Sebastes schlegelii tetracycline hydrochloride(TC) alizarin complexone(ALC) otoliths scales fin spines |
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