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Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece sp. 113
作者姓名:苏传东  池振明  卢伟东
作者单位:UNESCO Chinese Center of Marine Biotechnology Ocean University of China,UNESCO Chinese Center of Marine Biotechnology Ocean University of China,UNESCO Chinese Center of Marine Biotechnology Ocean University of China,Qingdao 266003 China Department of Biological Engineering Vocational Institute of Zibo Zibo 255314 China,Qingdao 266003 China,Qingdao 266003 China
摘    要:Cyanothece sp. 113 is a unicellular, aerobic, diazotrophic and photosynthetic marine cyanobacterium. The optimal medium for exopolysaccharide yield by the strain was 70.0 g/L of NaCl, and 0.9 g/L of MgSO4 based on the modified F/2 medium for cultivation of marine algae. The optimal cultivation condition for exopolysaccharide yield by this cyanobacterial strain was 29°C, aeration, and continuous illumination at 86.0 μE/M2/S. Under the optimal conditions, over 18.4 g/L of exopolysaccharide was produced within 12 days. This was so far the highest exopolysaccharide yield produced with strains of Cyanothece sp. obtained.

关 键 词:固氮作用  光合作用  海洋蓝青藻  生长特性
收稿时间:8 May 2006
修稿时间:2006-05-08

Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine <Emphasis Type="Italic">Cyanothece</Emphasis> sp. 113
Su Chuandong,Chi Zhenming,Lu Weidong.Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece sp. 113[J].Chinese Journal of Oceanology and Limnology,2007,25(4):411-417.
Authors:Su Chuandong  Chi Zhenming  Lu Weidong
Institution:UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao 266003 China; Department of Biological Engineering, Vocational Institute of Zibo, Zibo 255314, China
Abstract:Cyanothece sp. 113 is a unicellular, aerobic, diazotrophic and photosynthetic marine cyanobacterium. The optimal medium for exopolysaccharide yield by the strain was 70.0 g/L of NaCl, and 0.9 g/L of MgSO4 based on the modified F/2 medium for cultivation of marine algae. The optimal cultivation condition for exopolysaccharide yield by this cyanobacterial strain was 29°C, aeration, and continuous illumination at 86.0 μE/M2/S. Under the optimal conditions, over 18.4 g/L of exopolysaccharide was produced within 12 days. This was so far the highest exopolysaccharide yield produced with strains of Cyanothece sp. obtained. Supported by the NSFC (No. 30328021)
Keywords:Cyanothece  exopolysaccharide  N2 fixation  photosynthesis  marine cyanobacteria
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