| Application of quantitative PCR method in detection of Lymphocystis disease virus China （LCDV-cn） in Japanese flounder （Paralichthys olivaceus） |
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| 引用本文： | 昝金东,孙修勤,张之文,曲凌云,张进兴.Application of quantitative PCR method in detection of Lymphocystis disease virus China （LCDV-cn） in Japanese flounder （Paralichthys olivaceus）[J].中国海洋与湖沼学报,2007,25(4):418-422. |
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| 作者姓名： | 昝金东 孙修勤 张之文 曲凌云 张进兴 |
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| 作者单位： | First institute of Oceanography SOA，First institute of Oceanography SOA，First institute of Oceanography SOA，First institute of Oceanography SOA，First institute of Oceanography SOA，Qingdao 266061 China，Qingdao 266061 China，Qingdao 266061 China，Qingdao 266061 China，Qingdao 266061 China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104virions/ml. No virus was found in the negative samples of flounder.
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| 关 键 词： | 淋巴疾病 病毒数量 鱼病 病症 |
| 收稿时间： | 18 August 2006 |
| 修稿时间： | 2006-08-18 |
Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (<Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>) |
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| Zan?Jindong,Sun?Xiuqin,Zhang?Zhiwen,Qu?Lingyun,Zhang?Jinxing.Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)[J].Chinese Journal of Oceanology and Limnology,2007,25(4):418-422. |
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| Authors: | Zan Jindong Sun Xiuqin Zhang Zhiwen Qu Lingyun Zhang Jinxing |
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| Institution: | First institute of Oceanography, SOA, Qingdao 266061, China |
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| Abstract: | Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104virions/ml. No virus was found in the negative samples of flounder. |
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| Keywords: | Lymphocystis Disease Quantitative PCR Virus Amount |
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