| Identification and characterization of pathogen to bacterial septicaemia in cultured turbot, Scophthalmus maximus |
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| 作者姓名： | 秦国民 张晓君 陈翠珍 房海 战文斌 |
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| 作者单位： | Key Laboratory of Oceanic Biotechnology of Jiangsu Huaihai Institute of Technology，Key Laboratory of Oceanic Biotechnology of Jiangsu Huaihai Institute of Technology，Department of Animal Science Hebei Normal University of Science and Technology，Department of Animal Science Hebei Normal University of Science and Technology，The MOE Key Laboratory of Mariculture Ocean University of China，Lianyungang 222005，China，Lianyungang 222005，China，Qinhuangdao 066600，China，Qinhuangdao 066600，China，Qingdao 266003 China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);河北省科技厅科研项目 |
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| 摘 要： | Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic information of pathogenic bacteria was studied, including morphology, physiological and biochemical characteristics, and the mol% G C of the DNA. In addition, representative strains (S010623-1, LH031120-1) were selected for molecular identification by partial 16S rRNA gene sequencing. The results show that the isolates (LH031120-1 to LH031120-6, HT040308-1 to HT040308-6, HT040620-1 to HT040620-6) from three farms were identified as Edwardsiella tarda. The isolates (S010610-1 to S010610-10, S010623-1 to S010623-20) from one farm were identified as Listonella anguillarum. We conducted studies on the pathogenicity of isolates by artificial infection, and revealed all infected groups in morbidity and mortality. The septicaemia infected turbot showed a syndrome similar to that of the naturally infected fish. Antibiotic sensitivity showed that of 37 antimicrobial agents, E. tarda was sensitive to 27 agents, and L. anguillarum was sensitive to 21 agents.
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| 关 键 词： | 大比目鱼 败血病 病原体 病理特征 |
| 收稿时间： | 24 February 2006 |
| 修稿时间： | 2006-02-24 |
Identification and characterization of pathogen to bacterial septicaemia in cultured turbot, <Emphasis Type="Italic">Scophthalmus maximus</Emphasis> |
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| Qin Guomin,Zhang Xiaojun,Chen Cuizhen,Fang Hai,Zhan Wenbin.Identification and characterization of pathogen to bacterial septicaemia in cultured turbot, Scophthalmus maximus[J].Chinese Journal of Oceanology and Limnology,2007,25(4):403-410. |
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| Authors: | Qin Guomin Zhang Xiaojun Chen Cuizhen Fang Hai Zhan Wenbin |
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| Institution: | Key Laboratory of Oceanic Biotechnology of Jiangsu, Huaihai Institute of Technology Lianyungang, 222005 China;Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600,China;The MOE Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic information of pathogenic bacteria was studied, including morphology, physiological and biochemical characteristics, and the mol% G C of the DNA. In addition, representative strains (S010623-1, LH031120-1) were selected for molecular identification by partial 16S rRNA gene sequencing. The results show that the isolates (LH031120-1 to LH031120-6, HT040308-1 to HT040308-6, HT040620-1 to HT040620-6) from three farms were identified as Edwardsiella tarda. The isolates (S010610-1 to S010610-10, S010623-1 to S010623-20) from one farm were identified as Listonella anguillarum. We conducted studies on the pathogenicity of isolates by artificial infection, and revealed all infected groups in morbidity and mortality. The septicaemia infected turbot showed a syndrome similar to that of the naturally infected fish. Antibiotic sensitivity showed that of 37 antimicrobial agents, E. tarda was sensitive to 27 agents, and L. anguillarum was sensitive to 21 agents. |
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| Keywords: | Turbot (Scophthalmus maximus L ) Edwardsiella tarda Listonella anguillarum Characterization septicaemia |
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