| DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTI—IIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS |
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| 引用本文： | 刘建国,殷明炎,张京浦,刘伟,孟昭才.DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTI—IIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS[J].中国海洋与湖沼学报,2002,20(4):358-364. |
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| 作者姓名： | 刘建国 殷明炎 张京浦 刘伟 孟昭才 |
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| 作者单位： | R.DCenterofMarineBiotechnology,InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China |
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| 摘 要： | This study on dynamic changes of culture color,astaxanthin and chlorophylls,inorganic N including N-NO3^-,N-NO2^- and N-NH4^ in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentration showed(1)ast/chl ratio was over 0.8 for brown and red algae,but was usually less than 0.5 for green and yellow algae;(2)N-NO3^-,in general,was unstable and decreased,except for a small unexpected increase in nitrate enriched treatment groups;(3)measurable amounts of N-NO2^- and N-NH4^ were observed respectively with three change modes although no extemal nitrite and ammonia were added into the culture;(4)a non-linear correlation between ast/chl ratio(or color)changes and the levels of N-NO3^-,N-NO2^-,N-NH4^ in H.pluvialis culture;(5)up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown(or red)when N-NO3^-,N-NO2^- and N-NH4^ fluctuated around 30,5,5μmol/L respectively;(6)existence of three dynamic modes of N-NO3^-,N-NO2^- and N-NH4^ changes,obviously associated with initial extemal nitrate;(7)the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L;and(8)0.5-10mmol/L of nitrate was theoretically conducive to cell growth in batch culture.
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| 关 键 词： | 绿藻 无机氮源 动态变化 叶绿素 亚硝酸盐 硝酸盐 氨 虾青素形成 海水 |
| 收稿时间： | 26 July 2000 |
| 修稿时间： | 24 February 2001 |
Dynamic changes of inorganic nitrogen and astaxanthin accumulation inHaematococcus pluvialis |
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| Liu Jian-guo,Yin Ming-yan,Zhang Jing-pu,Liu Wei,Meng Zhao-cai.Dynamic changes of inorganic nitrogen and astaxanthin accumulation inHaematococcus pluvialis[J].Chinese Journal of Oceanology and Limnology,2002,20(4):358-364. |
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| Authors: | Liu Jian-guo Yin Ming-yan Zhang Jing-pu Liu Wei Meng Zhao-cai |
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| Institution: | (1) R&D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China |
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| Abstract: | This study on dynamic changes of culture color, astaxanthin and chlorophylls, inorganic N including N−NO
3
−
, N−NO
2
−
and N−NH
4
+
in batch culture ofHaematococcus pluvialis exposed to different additive nitrate concentration showed (1) ast/chl ratio was over 0.8 for brown and red algae, but was
usually less than 0.5 for green and yellow algae; (2) N−NO
3
−
, in general, was unstable and decreased, except for a small unexpected increase in nitrate enriched treatment groups; (3)
measurable amounts of N−NO
2
−
, and N−NH
4
+
were observed respectively with three change modes although no external nitrite and ammonia were added into the culture;
(4) a non-linear correlation between ast/chl ratio (or color) changes and the levels of N−NO
3
−
, N−NO
2
−
, N−NH
4
+
inH. pluvialis culture; (5) up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow
to brown (or red) when N−NO
3
−
, N−NO
2
−
and N−NH
4
+
fluctuated around 30, 5, 5, μmol/L respectively; (6) existence of three dynamic modes of N−NO
3
−
, N−NO
2
−
and N−NH
4
+
changes, obviously associated with initial external nitrate; (7) the key level of total inorganic N concentration regulating
the above physiological changes during indoor cultivation was about 50 μmol/L; and (8) 0.5–10 mmol/L of nitrate was theoretically
conducive to cell growth in batch culture.
Contribution No. 4139 from Institute of Oceanology, Chinese Academy of Sciences.
Projects 39500114, 39970575, A/2786-1 and 9565 supported by NSFC, IFS (International Foundation for Sciences) and Sci-Tech
Commission of Shandong Province respectively. |
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| Keywords: | Haematococcus pluvialis nitrate nitrite ammonia astaxanthin |
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