Screen and effect analysis of immunostimulants for sea cucumber,Apostichopus japonicus |
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| Authors: | LI Jiye SUN Xiuqin ZHENG Fengrong HAO Linhua College of Marine Life Science Ocean University of China Qingdao China North China Sea Environmental Monitoring Center State Oceanic Administration Qingdao China The First Institute of Oceanography SOA Qingdao China |
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| Institution: | [1]College of Marine Life Science, Ocean University of China, Qingdao 266003, China [2]North China Sea Environmental Monitoring Center, State Oceanic Administration, Qingdao 266033, China [3]The First Institute of Oceanography, SOA, Qingdao 266061, China |
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| Abstract: | Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal's coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides (P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day 1 and day 2 after the injection (P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection (P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus. |
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| Keywords: | Apostichopus japonicus immunostimulant lysozyme superoxide dismutase |
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