Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara |
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| Authors: | Qingpi Yan Minhui Zhao Xiaolu Wang Wenzheng Zou Changsheng Chen |
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| Institution: | 1. Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College of Jimei University, Xiamen, 361021, China
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| Abstract: | Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria
with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering
bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus
at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was
detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial
adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations
enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus,
and the environment. |
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