| Purification and characterization of lectin from humoral fluids of Charybdis feriatus |
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| 作者姓名： | 戴聪杰 王桂忠 何剑锋 李少菁 黄辉洋 |
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| 作者单位： | Department of Oceanography State key Lab of Marine Environmental Sciences Xiamen University，Department of Oceanography State key Lab of Marine Environmental Sciences Xiamen University，Polar Research Institute of China，Department of Oceanography State key Lab of Marine Environmental Sciences Xiamen University，Department of Oceanography State key Lab of Marine Environmental Sciences Xiamen University，Xiamen 361005 China Polar Research Institute of China Shanghai 200136 China Department of Biology Quanzhou Normal College Quanzhou 362000 China，Xiamen 361005 China，Shanghai 200136 China，Xiamen 361005 China，Xiamen 361005 China |
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| 基金项目： | Supported by Project of Technology Bureau of Quanzhou of Fujian Province(Z200239) , and by the Fujian Natural Science Foundation (B0410027) |
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| 摘 要： | 1 INTRODUCTION Lectins have been regarded as having a puta- tive role in non-self recognition in vertebrate and invertebrate immunities (Arason, 1996; Matsushita, 1996; Olafsen, 1996; Vasta et al., 1999; Wilson, et al., 1999; Marques and Barracco, 2000). …
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| 关 键 词： | 硫酸铵 纯化 凝集作用 CFL 海水化学 |
| 收稿时间： | 2005-06-14 |
| 修稿时间： | 2006-07-18 |
Purification and characterization of lectin from humoral fluids ofCharybdis feriatus |
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| Dai Congjie,Wang Guizhong,He Jianfeng,Li Shaojing,Huang Huiyang.Purification and characterization of lectin from humoral fluids of Charybdis feriatus[J].Chinese Journal of Oceanology and Limnology,2006,24(4):390-394. |
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| Authors: | Dai Congjie Wang Guizhong He Jianfeng Li Shaojing Huang Huiyang |
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| Institution: | (1) Department of Oceanography, State key Lab of Marine Environmental Sciences, Xiamen University, 361005 Xiamen, China;(2) Polar Research Institute of China, 200136 Shanghai, China;(3) Department of Biology Quanzhou Normal College, 362000 Quanzhou, China |
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| Abstract: | To search new sources of lectin, an experiment on lectin distribution in humoral serum of crabCharybdis feriatus (in short, CFL) was conducted March, 2002. When adding solid ammonium sulfate into the fluids up to 50% saturation at 4°C,
most CFL activity showed precipitates who were then continually extracted by ammonium sulfate of different concentrations.
The supernatant, which was called primary CFL fluids, was given a 17.60-fold purification and 45.70% recovery of total activity.
Finally, by using Sephadex G-100 column chromatography, the CFL in the primary CFL fluid was highly purified. Compared to
the original humoral fluids, the last purified CFL got a 203.90-fold purification and 30.48% recovery of total activity, and
demonstrated a single band on SDS-PAGE. In the same time, the purified CFL was detected for agglutination activity with 7
kinds of animal erythrocytes. Other characterization, such as sugar inhibition, and the effect of temperature on the agglutination
activity of CFL were also studied. Our results indicate that agglutination activity of CFL was influenced by sugar and temperature.
Supported by Project of Technology Bureau of Quanzhou of Fujian Province (Z200239), and by the Fujian Natural Science Foundation
(B0410027) |
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| Keywords: | ammonium sulfate purification CFL (lectin from Charybdis feriatus) characterization |
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