| 摘 要： | In this study,we conducted investigations in the Changjiang(Yangtze) River estuary and adjacent waters(CREAW) in June and November of 2014.We collected water samples from different depths to analyze the nitrogen isotopic compositions of nitrate,nutrient concentrations(including inorganic N,P,and Si),and other physical and biological parameters,along with the vertical distribution and seasonal variations of these parameters.The compositions of nitrogen isotope in nitrate were measured with the denitrifier method.Results show that the Changjiang River diluted water(CDW) was the main factor affecting the shallow waters(above 10 m) of the CREAW,and CDW tended to influence the northern areas in June and the southern areas in November.δ(15)N_(NO_3) values in CDW ranged from 3.21‰-3.55‰.In contrast,the deep waters(below 30 m) were affected by the subsurface water of the Kuroshio Current,which intruded into the waters near 31°N in June.The δ(15)N_(NO_3) values of these waters were 6.03‰-7.6‰,slightly higher than the values of the Kuroshio Current.Nitrate assimilation by phytoplankton in the shallow waters of the study area varied seasonally.Because of the favorable temperature and nutrient conditions in June,abundant phytoplankton growth resulted in harmful algae blooms(HABs).Therefore,nitrate assimilation was strong in June and weak in November.The δ(15)N_(NO_3) fractionations caused by assimilation of phytoplankton were4.57‰ and 4.41‰ in the shallow waters in June and November,respectively.These results are consistent with previous laboratory cultures and in situ investigations.Nitrification processes were observed in some deep waters of the study area,and they were more apparent in November than in June.The fractionation values of nitrification ranged from 24‰-25‰,which agrees with results for Nitrosospira tenuis reported by previous studies.
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