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Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea |
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| Authors: | Shujiang Zhao Shuping Li Huihui Liu Qian Zhao Jieyou Wang Maocang Yan |
| Institution: | 1. School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou, 325035, China 2. Department of Pharmacy, Zhengzhou Huaxin College, Zhengzhou, 451150, China 3. Mariculture Institute of Zhejiang, Wenzhou, 325005, China |
| Abstract: | Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island, Wenzhou, Zhejiang Province, China. Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic V ibrio from P seudosciaena crocea. Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic V ibrio. Strain NJ0104 had the widest antimicrobial spectrum and strong activity, particularly against Vibrio parahaemolyticus-MM0810072. A preliminary study of NJ0104 antibacterial metabolites demonstrated that they had thermal stability up to 80°C, ultraviolet stability up to 40 min and pH stability between 4.0–7.0. In addition, the antibacterial metabolites were readily soluble in butanol. To identify the specific strain, the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced. Based on the combination of phenotypic and genotypic data, the strain was identified as Arthrinium sp. |
| Keywords: | Nanji Island marine fungi activity stability ITS-5 8S rDNA |
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