| UP-REGULATION OF HEPATIC RECEPTOR FOR GROWTH HORMONE IN THE FLOUNDER （PARALICHTHYS OLIVACEUS） AFTER ORAL ADMINISTRATION WITH EXOGENOUS GH |
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| 引用本文： | 刘宗柱,王金宝,徐永立,王勇,张培军.UP-REGULATION OF HEPATIC RECEPTOR FOR GROWTH HORMONE IN THE FLOUNDER （PARALICHTHYS OLIVACEUS） AFTER ORAL ADMINISTRATION WITH EXOGENOUS GH[J].中国海洋与湖沼学报,2001,19(2):135-140. |
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| 作者姓名： | 刘宗柱 王金宝 徐永立 王勇 张培军 |
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| 作者单位： | [1]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China [2]Dep.Anim.Sci.LaiyangAgricultureCollege,Laiyang265200 |
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| 基金项目： | Project 96 C0 1 0 5 0 4supportedbyNational“NinthFiveYearPlanKeyProblem” . |
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| 摘 要： | The iodination efficiency of salmon GH (sGH) was 38.82%,using a modification of the chloramine-T method. The specific activity of the ^125I-sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low-capacity binding site in flounder liver. Long-term administration with exogenous GH can induce the up-regulation of hepatic GH receptor in total binding capacity though there was no significant difference of association constant among any groups. Con-sidering that there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites.
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| 关 键 词： | 碘 生长激素 荷尔蒙 肝脏 受体 调节 |
Up-regulation of hepatic receptor for growth hormone in the flounder (Paralichthys olivaceus) after oral administration with exogenous GH |
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| Zong-zhu Liu,Jin-bao Wang,Yong-li Xu,Yong Wang,Pei-jun Zhang.Up-regulation of hepatic receptor for growth hormone in the flounder (Paralichthys olivaceus) after oral administration with exogenous GH[J].Chinese Journal of Oceanology and Limnology,2001,19(2):135-140. |
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| Authors: | Zong-zhu Liu Jin-bao Wang Yong-li Xu Yong Wang Pei-jun Zhang |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Dep. Anim. Sci. Laiyang Agriculture College, 265200 Laiyang |
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| Abstract: | The iodination efficiency of salmon GH(sGH) was 38.82%, using a modification of the chloramine-T method. The specific activity
of the125I-sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low-capacity binding
site in flounder liver. Long-term administration with exogenous GH can induce the up-regulation of hepatic GH receptor in
total binding capacity though there was no significant difference in capacity of free binding sites of livers from control
and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish,
had more occupied binding sites.
Contribution No.247 of Experimental Marine Biology Laboratory and 3798 of the Institute of Oceanology, Chinese Academy of
Sciences.
Project 96-C01-05-04 supported by National “Ninth Five Year Plan Key Problem”. |
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| Keywords: | iodination GH hepatic receptor up regulation |
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