Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch |
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| Authors: | Huicheng Yang Mingyong Zeng Shiyuan Dong Zunying Liu Ruixue Li |
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| Institution: | (1) Institute for Polar Ecology, University of Kiel, Wischhofstr. 1-3, Building 12, 24148 Kiel, Germany;(2) Instituto de Biologia Marina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile;(3) Alfred Wegener Institute for Polar and Marine Research, Marine Station, 27498 Helgoland, Germany;(4) Biocenter Klein-Flottbek, University of Hamburg, 22609 Hamburg, Germany;(5) Institute of Biological Sciences, Applied Ecology, University of Rostock, 18059 Rostock, Germany;(6) Alfred Wegener Institute for Polar and Marine Research, 27570 Bremerhaven, Germany |
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| Abstract: | In this study, we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay. Cells
were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h. The inhibitory rate of PE on BEL-7402 and P388 cells
was 30.20±1.16% and 43.44±1.86%, respectively, and the half-inhibitory concentration of PE (IC50) on P388 and BEL-7402 cells was 120 μg/mL and >200 μg/mL, respectively. Microscopic observation shows that the morphologic
features of tumor cells treated with PE and 5-fluorouracil are markedly different from the normal control group. The inhibitory
rate of fraction A2 isolated from PE by sephadex LH-20 for BEL-7402 and P388 cells at the sample concentration of 70.42 μg/mL was 61.96±7.02%
and 40.47±8.70%, respectively. The apoptosis peak for fraction A2 was the most profound of all fractions used in the flow cytometry assay. The results indicate that the anti-proliferative
of this algal extract is associated with the total phlorotannin content. |
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