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Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease |
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| Authors: | MO Zhaolan GUO Dongsheng MAO Yunxiang YE Xuhong ZOU Yuxia XIAO Peng HAO Bin Institute of Oceanology Chinese Academy of Sciences Qingdao China Graduate University of Chinese Academy of Sciences Beijing China Ocean University of China Qingdao China |
| Institution: | MO Zhaolan,GUO Dongsheng,MAO Yunxiang,YE Xuhong,ZOU Yuxia,XIAO Peng,HAO Bin Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China Graduate University of Chinese Academy of Sciences,Beijing 100039,China Ocean University of China,Qingdao 266003,China |
| Abstract: | We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In additi... |
| Keywords: | Vibrio anguillarum prtV proteolysis hemolysis mucus LD50 |
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