| Free radical scavenging abilities of polypeptide from Chlamys farreri |
| |
| 作者姓名： | 韩志武 初晓 刘成娟 王跃军 孙谧 王春波 |
| |
| 作者单位： | Affiliated Hospital of Medical School Qingdao University Qingdao 266003 China，Affiliated Hospital of Medical School Qingdao University Qingdao 266003 China，Affiliated Hospital of Medical School Qingdao University Qingdao 266003 China，Research Institute of Yellow Sea Aquatic Products Qingdao 266032 China，Research Institute of Yellow Sea Aquatic Products Qingdao 266032 China，Research Institute of Yellow Sea Aquatic Products Qingdao 266032 China |
| |
| 基金项目： | Supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology Bureau of Qingdao (No. 2001-28-50). |
| |
| 摘 要： | 1 INTRODUCTION Active oxygen free radicals can damage cell structure, even kill the cells, causing aging and cer- tain serious diseases (Bors et al., 1989). Usually, the production and scavenging of active oxygen free radicals are in balance in healthy hu…
|
| 关 键 词： | 多肽 自由基 抗氧化剂 化学发光 电子自旋 |
| 收稿时间： | 2005-01-04 |
| 修稿时间： | 2005-08-26 |
Free radical scavenging abilities of polypeptide fromChlamys farreri |
| |
| Han Zhiwu,Chu Xiao,Liu Chengjuan,Wang Yuejun,Sun Mi,Wang Chunbo.Free radical scavenging abilities of polypeptide from Chlamys farreri[J].Chinese Journal of Oceanology and Limnology,2006,24(3):325-328. |
| |
| Authors: | Han Zhiwu Chu Xiao Liu Chengjuan Wang Yuejun Sun Mi Wang Chunbo |
| |
| Institution: | (1) Affiliated Hospital of Medical School, Qingdao University, 266003 Qingdao, China;(2) Research Institute of Yellow Sea Aquatic Products, 266032 Qingdao, China |
| |
| Abstract: | We investigated the radical scavenging effect and antioxidation property of polypeptide extracted fromChlamys farreri (PCF)in vitro using chemiluminescence and electron spin resonance (ESR) methods. We examined the scavenging effects of PCF on superoxide
anions (O
2
−
), hydroxyl radicals (OH·), peroxynitrite (ONOO−) and the inhibiting capacity of PCF on peroxidation of linoleic acid. Our experiment suggested that PCF could scavenge oxygen
free radicals including superoxide anions (O
2
−
) (IC50=0.3 mg/ml), hydroxyl radicals (OH·) (IC50=0.2 μg/ml) generated from the reaction systems and effectively inhibit the oxidative activity of ONOO− (IC50=0.2 mg/ml). At 1.25 mg/ml of PCF, the inhibition ratio on lipid peroxidation of linoleic acid was 43%. The scavenging effect
of PCF on O
2
−
, OH· and ONOO− free radicals were stronger than those of vitamin C but less on lipid peroxidation of linoleic acid. Thus PCF could scavenge
free radicals and inhibit the peroxidation of linoleic acidin vitro. It is an antioxidant from marine products and potential for industrial production in future.
Supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology Bureau of Qingdao
(No. 2001-28-50). |
| |
| Keywords: | polypeptide Chlamys farreri free radical antioxidant chemiluminescence electron spin resonance (ESR) |
| 本文献已被 CNKI 万方数据 SpringerLink 等数据库收录！ |
