Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge |
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| Authors: | CONG Ming L Jiasen WU Huifeng ZHAO Jianmin |
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| Institution: | 1. Key Laboratory of Coastal Zone Environment Processes, Chinese Academy of Sciences; Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003,China
2. Biology School of Yantai University, Yantai 264005, China |
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| Abstract: | Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium (Cd) on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), acid phosphatase (ACP), Na+, K+ -ATPase in gills and hepatopancreas, and phenoloxidase-like (POL) enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 (HSP90), metallothionein (MT), and bactericidal/permeability increasing (BPI) protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na+, K+-ATPase activities in both tissues, and MT expression was increased in Cdexposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters’ immune responses and energy metabolism following vibrio infection. |
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| Keywords: | cadmium Listonella anguillarum Crassostrea gigas defensive enzyme gene expression |
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