| Study of light signal receptor of Stephanopyxis palmeriana during sexual reproduction |
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| 作者姓名： | 胡韧 林均民 林秋奇 韩博平 |
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| 作者单位： | [1]ResearchCenterofHydrobiology,JinanUniversity,Guangzhou510632,China／／SchoolofLifeScience.XiamenUniversity,Xiamen361005,China [2]SchoolofLifeScience.XiamenUniversity,Xiamen361005,China [3]ResearchCenterofHydrobiology,JinanUniversity,Guangzhou510632,China |
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| 基金项目： | Supported by National Natural Science Foundation of China (No. 39670076) |
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| 摘 要： | We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for characteristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual reproduction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of S.palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of S. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of S. palmeriana is phytochrome B (phyB).
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| 关 键 词： | 硅藻 有性生殖 光周期 信号接受器 |
Study of light signal receptor of Stephanopyxis palmeriana during sexual reproduction |
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| HU Ren,LIN Junmin,LIN Qiuqi,HAN Boping Research Center of Hydrobiology,Jinan University,Guangzhou ,China School of Life Science,Xiamen University,Xiamen ,China.Study of light signal receptor of Stephanopyxis palmeriana during sexual reproduction[J].Chinese Journal of Oceanology and Limnology,2005,23(3):330-334. |
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| Authors: | HU Ren LIN Junmin LIN Qiuqi HAN Boping Research Center of Hydrobiology Jinan University Guangzhou China School of Life Science Xiamen University Xiamen China |
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| Institution: | HU Ren,LIN Junmin,LIN Qiuqi,HAN Boping Research Center of Hydrobiology,Jinan University,Guangzhou 510632,China School of Life Science,Xiamen University,Xiamen 361005,China |
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| Abstract: | We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for charac- teristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual repro- duction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of S. palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of S. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of S. palmeriana is phytochrome B (phyB). |
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| Keywords: | diatom sexual reproduction photoperiod light signal receptor |
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