Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus |
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| Authors: | Ye Yuan Xiuli Wang Sheping Guo Xuemei Qiu |
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| Institution: | YUAN Ye,WANG Xiuli **,GUO Sheping,QIU Xuemei College of Life Sciences and Biotechnology,Dalian Ocean University,Dalian 116023,China |
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| Abstract: | Gram-negative Vibrio parahaemolyticus is a common pathogen in humans and marine animals. The outer membrane protein of bacteria plays an important role in the infection and pathogenicity to the host. Thus, the outer membrane proteins are an ideal target for vaccines. We amplified a complete outer membrane protein gene (ompW) from V. parahaemolyticus ATCC 17802. We then cloned and expressed the gene into Escherichia coli BL21 (DE3) cells. The gene coded for a protein that was 42.78 kDa. We purified the prote... |
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| Keywords: | Vibrio parahaemolyticus outer membrane protein (OmpW) cloning prokaryotic expression protein characterization recombinant proteins |
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