| cDNA cloning and expression of a collectin from red-spotted grouper （Epinephelus akaara） |
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| 引用本文： | 张之文,丁少雄,王颖,毛勇,苏永全,王军.cDNA cloning and expression of a collectin from red-spotted grouper （Epinephelus akaara）[J].中国海洋与湖沼学报,2009,27(3):543-549. |
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| 作者姓名： | 张之文 丁少雄 王颖 毛勇 苏永全 王军 |
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| 作者单位： | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China |
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| 基金项目： | Supported by the Natural Science Foundation of Fujian Province of China (No 2060203);;New Century Excellent Talents supporting funding of Fujian Province |
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| 摘 要： |
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| 关 键 词： | cDNA克隆 石斑鱼 胶凝 甘露聚糖结合凝集素 cDNA序列 重组表达载体 C型凝集素 无脊椎动物 |
| 收稿时间： | 27 March 2008 |
cDNA cloning and expression of a collectin from red-spotted grouper (<Emphasis Type="Italic">Epinephelus akaara</Emphasis>) |
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| Zhiwen?Zhang,Shaoxiong?Ding,Ying?Wang,Yong?Mao,Yongquan?Su,Jun?Wang.cDNA cloning and expression of a collectin from red-spotted grouper (Epinephelus akaara)[J].Chinese Journal of Oceanology and Limnology,2009,27(3):543-549. |
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| Authors: | Zhiwen Zhang Shaoxiong Ding Ying Wang Yong Mao Yongquan Su Jun Wang |
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| Institution: | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China |
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| Abstract: | Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens.We obtained the complete cDNA of a C-type lectin(EALec1) from Epinephelus akaara using RACE.The complete EALec1 cDNA sequence was 827 bp.The 5-UTR and 3-UTR were 28 bp and 151 bp,respectively,in length.The sequence also contained a polyadenylation signal AATAAA and a poly(A) tail.The EALec1 cDNA encodes polypeptides with 215 amino acids,including a signal peptide of 31 amino acids.Th... |
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| Keywords: | Epinephelus akaara collectin expression clone |
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