| Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafish Danio Rerio |
| |
| 作者姓名： | SAWANT M. S 张士璀 王清印 |
| |
| 作者单位： | [1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China [2]Yellow Sea Fisheries Research Institute, Qingdao 266071, China |
| |
| 基金项目： | This work was supported by Ministry of Science and Technology （M0ST） of China （G 1999012005）. |
| |
| 摘 要： | Zebrafish （Danio rerio） genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine, while the late development （organogenesis） was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail, wavy body, golden pigmentation and the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures, curled tails and wavy body. RAPD analysis showed that the majority of 5-bromouracil-and 6-aminopurine-treated larvae and fish shared banding patterns in common with the control, suggesting that most mutagenesis induced by these agents are point mutations. However, some fish derived from 5-bromouracil-treated embryos had golden（gol） pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control.Possibly, 5-bromouracil can occasionally cause relatively extensive changes in the fish genome. The results of this study may provide valuable help for detailed studies of mutagenesis.
|
| 关 键 词： | 溴尿嘧啶 氨基嘌呤 斑鱼 突变反应 |
| 收稿时间： | 2003-09-16 |
| 修稿时间： | 2004-03-15 |
Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafish Danio Rerio |
| |
| SAWANT;M.;S;Zhang ShiCui;Wang QingYin.Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafish Danio Rerio[J].Chinese Journal of Oceanology and Limnology,2005,23(1):54-59. |
| |
| Authors: | SAWANT;M;S;Zhang ShiCui;Wang QingYin |
| |
| Abstract: | Zebrafish (Danio rerio) genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine, while the late development (organogenesis) was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail, wavy body, golden pigmentation and the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures, curled tails and wavy body. RAPD analysis showed that the majority of 5-bromouracil- and 6-aminopurine-treated larvae and fish shared banding patterns in common with the control, suggesting that most mutagenesis induced by these agents are point mutations. However, some fish derived from 5-bromouracil-treated embryos had golden (gol) pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control. Possibly, 5-bromouracil can occasionally cause relatively extensive changes in the fish genome. The results of this study may provide valuable help for detailed studies of mutagenesis. |
| |
| Keywords: | zebrafish Danio rerio 5-bromouracil 6-aminopurine mutagenesis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
