Screen and effect analysis of immunostimulants for sea cucumber, <Emphasis Type="Italic">Apostichopus japonicus</Emphasis> |
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| Authors: | Jiye Li Xiuqin Sun Fengrong Zheng Linhua Hao |
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| Institution: | (1) College of Marine Life Science, Ocean University of China, Qingdao, 266003, China;(2) North China Sea Environmental Monitoring Center, State Oceanic Administration, Qingdao, 266033, China;(3) The First Institute of Oceanography, SOA, Qingdao, 266061, China |
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| Abstract: | Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the
organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide
and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides
were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were
monitored in six consecutive days. The results show that the lysozyme activity of the animal’s coelomic fluid was significantly
stimulated on day 2, day 4 and day 6 after the injection of the saccharides (P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime
was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day
1 and day 2 after the injection (P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection
(P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast
polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.
Supported by the National Key Technology Research and Development Program (863 Program, No. 2006BAD09A06) and the Special
Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes (No. 02-2007B03) |
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| Keywords: | Keyword" target="_blank">Keyword Apostichopus japonicus immunostimulant lysozyme superoxide dismutase |
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