Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine <Emphasis Type="Italic">Arthrobacter oxydans</Emphasis> KQ11 dextranase mouthwash |
| |
| Authors: | Wei Ren Shujun Wang Mingsheng Lü Xiaobei Wang Yaowei Fang Yuliang Jiao Jianen Hu |
| |
| Institution: | 1.College of Food Science and Engineering,Dalian Ocean University,Dalian,China;2.Jiangsu Marine Resources Development Research Institute,Lianyungang,China;3.Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,Huaihai Institute of Technology,Lianyungang,China;4.Co - Innovation Center of Jiangsu Marine Bio - Industry Technology,Huaihai Institute of Technology,Lianyungang,China |
| |
| Abstract: | We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofilm formation by Streptococcus mutans, which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash (designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of ZnSO4, lysozyme, citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L ZnSO4, 14 g/L lysozyme, 4.5 g/L citric acid and 5 g/L chitosan. The biofilm formation inhibition reached 84.49%. In addition, microscopic observation of the biofilm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S. mutans. This work may be promoted for the design and development of future marine dextranase oral care products. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
