| Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus： karyotype, allozyme and RAPD analyses |
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| 引用本文： | 尤锋,王伟,徐冬冬,朱香萍,倪静,吴志昊,徐永立,王新成,张培军.Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus： karyotype, allozyme and RAPD analyses[J].中国海洋与湖沼学报,2009,27(2):317-323. |
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| 作者姓名： | 尤锋 王伟 徐冬冬 朱香萍 倪静 吴志昊 徐永立 王新成 张培军 |
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| 摘 要： |
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| 关 键 词： | RAPD分析 染色体核型 橄榄树 石鲽 牙鲆 杂交后代 杂种 酶 |
| 收稿时间： | 10 July 2008 |
Hybrids between olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis> and stone flounder <Emphasis Type="Italic">Kareius bicoloratus</Emphasis>: karyotype,allozyme and RAPD analyses |
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| Feng?You,Wei?Wang,Dongdong?Xu,Xiangping?Zhu,Jing?Ni,Zhihao?Wu,Yongli?Xu,Xincheng?Wang,Peijun?Zhang.Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses[J].Chinese Journal of Oceanology and Limnology,2009,27(2):317-323. |
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| Authors: | Feng You Wei Wang Dongdong Xu Xiangping Zhu Jing Ni Zhihao Wu Yongli Xu Xincheng Wang Peijun Zhang |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;(2) Qingdao Agricultural University, Qingdao, 266109, China |
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| Abstract: | The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types
of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species.
Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no
difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also
studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal
and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above
38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation
could inherit a little more genetic materials from paternal fish than that from maternal fish.
Supported by National Natural Science Foundation of China (No. 30571445), National High-Technology Research and Development
Program (863 Program, No. 2006AA10A404), and Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017) |
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| Keywords: | Keyword" target="_blank">Keyword artificial hybridization Paralichthys olivaceus (Temminck et Schlegel) Kareius bicoloratus (Basilewsky) karyotype allozyme RAPD |
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