Characterization of genic microsatellite markers derived from expressed sequence tags in Pacific abalone (Haliotis discus hannai) |
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| Authors: | Qi Li Jing Shu Cui Zhao Shikai Liu Lingfeng Kong Xiaodong Zheng |
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| Institution: | (1) Fisheries College, Ocean University of China, Qingdao, 266003, China;(2) Marine Field Station, Field Science Center, Graduate School of Agricultural Science, Tohoku University, Onagawa Oshika, 986-2242, Japan |
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| Abstract: | Simple sequence repeat (SSR) markers were developed from the expressed sequence tags (ESTs) of Pacific abalone (Haliotis discus hannai). Repeat motifs were found in 4.95% of the ESTs at a frequency of one repeat every 10.04 kb of EST sequences, after redundancy
elimination. Seventeen polymorphic EST-SSRs were developed. The number of alleles per locus varied from 2–17, with an average
of 6.8 alleles per locus. The expected and observed heterozygosities ranged from 0.159 to 0.928 and from 0.132 to 0.922, respectively.
Twelve of the 17 loci (70.6%) were successfully amplified in H. diversicolor. Seventeen loci segregated in three families, with three showing the presence of null alleles (17.6%). The adequate level
of variability and low frequency of null alleles observed in H. discus hannai, together with the high rate of transportability across Haliotis species, make this set of EST-SSR markers an important tool for comparative mapping, marker-assisted selection, and evolutionary
studies, not only in the Pacific abalone, but also in related species. |
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