The insemination of goldfish (Carassium auratus) oocyte maturedin vitro |
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| Authors: | Wang Renxue Wu Xianhan Zhou Jing Zhang Shicui Ma Yingjie Wu Shangqin Shi Yingxian |
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| Institution: | (1) Institute of Oceanology, Academia Sinica, 266071 Qingdao;(2) Institute of Developmental Biology, Academia Sinica, 100080 Beijing |
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| Abstract: | Full maturation of goldfish oocyte was inducedin vitro by 17 α-hydroxy-20β-dihydroprogesterone. The oocyte maturation involves GV migration to the periphery of the oocyte and germinal
vesicle breakdown (GVBD). In the experiment, incubation duration for GVBD varied in different broods of oocytes. Generally,
if the duration for GVBD was shorter than 6 h, oocytes would have a better chance to survive after maturation and insemination.
The maturation of nucleus (GV) and cytoplasm are not synchronous. Cytoplasm maturation occurs several hs after GVBD. Oocytes
inseminated 8–9 h after GVBD have the highest fertilizing and hatching rate. Fertilized ova maturedin vitro can develop to sexually mature adults capable of reproduction.
Contribution No 1731 from the Institute of Oceanology, Academia Sinica. |
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