Non-specific immune response of bullfrog <Emphasis Type="Italic">Rana catesbeiana</Emphasis> to intraperitoneal injection of bacterium <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> |
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| Authors: | Junjie Zhang Wenzheng Zou Qingpi Yan |
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| Institution: | (1) Fisheries College, Jimei University, Xiamen, 361021, China;(2) Chemistry and Chemical Engineering College of Xiamen University, Xiamen, 361005, China |
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| Abstract: | Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally (i.p.)
with 0.2 ml bacterial suspension at a density of 5.2 × 106 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution (0.85%, w/v). Three bullfrogs in
both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection (dpi) for the evaluation of non-specific immune
parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases
in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when
compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral
erythrocytes, serum superoxide dismutase (SOD) activity, and lysozyme activity was detected between the two groups. It is
suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection.
Supported by National High-Tech Research and Development Program of China (863 Program, No.2001AA5070, 2002AA639600) and Natural
Science Foundation of Fujian Province of China (No.B0410022, 2006F5066) |
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| Keywords: | Keyword" target="_blank">Keyword Rana catesbeiana Aeromonas hydrophila intraperitoneal injection non-specific immune response |
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