| Cloning of the cDNA encoding adenosine 5′-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus |
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| 引用本文： | 蒋克勇,孙姝娟,刘梅,王宝杰,孟晓林,王雷.Cloning of the cDNA encoding adenosine 5′-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus[J].中国海洋与湖沼学报,2013,31(1):118-127. |
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| 作者姓名： | 蒋克勇 孙姝娟 刘梅 王宝杰 孟晓林 王雷 |
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| 作者单位： | R&D Center of Marine Biotechnology,Institute of Oceanology,Chinese Academy of Sciences;Graduate University of Chinese Academy of Sciences |
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| 基金项目： | Supported by the National Natural Science Foundation of China (No.41206144);the National High Technology Research and Development Program of China (863 Program) (No. 2008AA100805) |
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| 摘 要： | AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5’-UTR of 78 bp, a 3’-UTR of 237 bp and an open reading frame (ORF) of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif (SLSTDDP) and an ATP-binding site sequence (EPLMEEYAIAAQVFK). Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle (750±40 g) compared with juvenile muscle (7.5±2 g) (P<0.05). HPLC analysis showed that the IMP content in adult muscle (3.35±0.21 mg/g) was also statistically significantly higher than in juvenile muscle (1.08±0.04 mg/g) (P<0.05). There is a direct relationship between the AMPD1 gene expression level and IMP content in the skeletal muscle of juvenile and adult flounders. These results may provide useful information for quality improvement and molecular breeding of aquatic animals.
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| 关 键 词： | AMPD1 cDNA cloning mRNA expression IMP skeletal muscle Japanese flounder |
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