| Genetic variation in two sea cucumber （Apostichopus japonicus） stocks revealed by ISSR markers |
| |
| 引用本文： | 姚冰,胡晓丽,包振民,陆维,胡景杰.Genetic variation in two sea cucumber （Apostichopus japonicus） stocks revealed by ISSR markers[J].中国海洋与湖沼学报,2007,25(1):91-96. |
| |
| 作者姓名： | 姚冰 胡晓丽 包振民 陆维 胡景杰 |
| |
| 作者单位： | Laboratory of Marine Genetics and Breeding (MGB) Division of Life Science and Technology Ocean University of China Qingdao 266003 China)，Laboratory of Marine Genetics and Breeding (MGB) Division of Life Science and Technology Ocean University of China Qingdao 266003 China)，Laboratory of Marine Genetics and Breeding (MGB) Division of Life Science and Technology Ocean University of China Qingdao 266003 China)，Laboratory of Marine Genetics and Breeding (MGB) Division of Life Science and Technology Ocean University of China Qingdao 266003 China)，Laboratory of Marine Genetics and Breeding (MGB) Division of Life Science and Technology Ocean University of China Qingdao 266003 China) |
| |
| 基金项目： | 国家自然科学基金;教育部海洋生物重点实验室基金 |
| |
| 摘 要： | Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks. Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diver- sity was 0.360 5 and 0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall genetic differentiation and the ge- netic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks, showing that the genetic structure was unitary for PL stock but complex for QD stock.
|
| 关 键 词： | 海参 遗传变异 分子标记 遗传多样性 海产动物 |
| 收稿时间： | 2005-08-18 |
| 修稿时间： | 2005-12-20 |
Genetic variation in two sea cucumber (<Emphasis Type="Italic">Apostichopus japonicus</Emphasis>) stocks revealed by ISSR markers |
| |
| Yao?Bing,Hu?Xiaoli,Bao?Zhenmin,Lu?Wei,Hu?Jingjie.Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers[J].Chinese Journal of Oceanology and Limnology,2007,25(1):91-96. |
| |
| Authors: | Yao Bing Hu Xiaoli Bao Zhenmin Lu Wei Hu Jingjie |
| |
| Institution: | (1) Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Technology, Ocean University of China, Qingdao, 266003, China |
| |
| Abstract: | Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong
Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks.
Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diversity was 0.360 5 and
0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands
was 92.2%, the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall
genetic differentiation and the genetic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using
the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between
the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks, showing that the genetic
structure was unitary for PL stock but complex for QD stock.
Supported by the NSFC (No. 30571417) and The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China
(No. 200426) |
| |
| Keywords: | sea cucumber Apostichopus japonicus ISSR stock genetic diversity |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
|
