| Effects of temperature, pH and NaC1 on protease activity in digestive tract of young turbot, Scophthalmus maximus |
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| 作者姓名： | 陈慕雁 张秀梅 高天翔 陈超 |
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| 作者单位： | [1]Fishery College, Ocean University of China, Qingdao 266003, China [2]Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| 基金项目： | Supported by the NSFC （No. 30070593）5 ACKN0WLEDGEMENT We sincerely thank Wan Zhenzhen for assistance during the experiment. |
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| 摘 要： | The protease activity in digestive tract of young turbot Scophthalmus maximum was studied, and the optimal pH, temperature and NaCl concentration were determined for different portions of the fish＇s internal organs. The optimal activity in the fish＇s stomach was at pH of 2.2, while that in the intestinal extracts was within the alkaline range from 9.5 to 10.0. In hepatopancreas, the optimal pH was in low alkalinity at 8.5. The optimal reaction temperature was above 40℃ in stomach, intestine and hepatopancreas. With increasing temperature, the pH value increased in stomach, while in the intestine, an opposite tendency was observed due to combined effect of pH and temperature. NaCl concentration showed inhibitory impact on protein digestion in hepatopancreas. The main protease for protein digestion in turbot seemed to be pepsin. Moreover, the maximum protease activity in different segments of intestine existed in the hindgut.
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| 关 键 词： | 比目鱼 温度 pH值 养殖环境 |
| 收稿时间： | 2005-01-04 |
| 修稿时间： | 2006-03-23 |
Effects of temperature, pH and NaCl on protease activity in digestive tract of young turbot,Scophthalmus maximus |
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| Chen Muyan,Zhang Xiumei,Gao Tianxiang,Chen Chao.Effects of temperature, pH and NaC1 on protease activity in digestive tract of young turbot, Scophthalmus maximus[J].Chinese Journal of Oceanology and Limnology,2006,24(3):300-306. |
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| Authors: | Chen Muyan Zhang Xiumei Gao Tianxiang Chen Chao |
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| Institution: | (1) Fishery College, Ocean University of China, 266003 Qingdao, China;(2) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071 Qingdao, China |
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| Abstract: | The protease activity in digestive tract of young turbotScophthalmus maximum was studied, and the optimal pH, temperature and NaCl concentration were determined for different portions of the fish's
internal organs. The optimal activity in the fish's stomach was at pH of 2.2, while that in the intestinal extracts was within
the alkaline range from 9.5 to 10.0. In hepatopancreas, the optimal pH was in low alkalinity at 8.5. The optimal reaction
temperature was above 40°C in stomach, intestine and hepatopancreas. With increasing temperature, the pH value increased in
stomach, while in the intestine, an opposite tendency was observed due to combined effect of pH and temperature. NaCl concentration
showed inhibitory impact on protein digestion in hepatopancreas. The main protease for protein digestion in turbot seemed
to be pepsin. Moreover, the maximum protease activity in different segments of intestine existed in the hindgut.
Supported by the NSFC (No. 30070593) |
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| Keywords: | turbot protease activity pH temperature NaCl |
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