| Assessing the Germplasm of Laminaria （Phaeophyceae） with Random Amplified Polymorphic DNA （RAPD） Method |
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| 引用本文： | 赫英俊,邹俞萍,王晓东,郑治国,张大明,段德麟.Assessing the Germplasm of Laminaria （Phaeophyceae） with Random Amplified Polymorphic DNA （RAPD） Method[J].中国海洋与湖沼学报,2003,21(2):141-148. |
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| 作者姓名： | 赫英俊 邹俞萍 王晓东 郑治国 张大明 段德麟 |
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| 作者单位： | [1]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China [2]InstituteofBotany,ChinsesAcademyofSciences,Beijing100093,China |
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| 基金项目： | ContributionNo.4 389fromtheInstituteofOceanology,hineseAcademyofSciences.ThisresearchwassupportedbytheState sHigh techProjectofChina,Marine 863project (No.81 9 0 3 0 2 )and
( 2 0 0 1AA62 1 0 90 ) . |
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| 摘 要： | Eighteen gametophytes including L.japonica ,L.ochotensis and L.longissima,were verified with random amplified polymorphic DNA (RAPD) technique.Eighteen ten-base primers were chosen from 100 primers selected for final amplification test.Among the total of 205 bands amplified ,181(88.3%) were polymorphic ,The genetic distance among different strains ranged from 0.072 to 0.391,The dendrogram constructed by unweighted pair -group method with arithmetic(UPGMA) method showed that the female and male gamtophytes of the same cell lines could be grouped in pairs respectively,It indicated that RAPD analysis could be used not only to distin-gusih different strains of Laminariz ,but also to distinguish male and female gametophyte within the same cell lines,There is ambiguous systematic relationship if judged mereely by the present data It seems that the use of RAPD marker is limited to elucidstion of the phylogenetic relationship among the species of Laminaria.
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| 关 键 词： | DNA 脱氧核糖核酸 RAPD 昆布属植物 配偶体 胚质 遗传变异 |
Assessing the Germplasm of Laminaria (Phaeophyceae) with Random Amplified PoLymorphic DNA (RAPD) Method |
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| Abstract: | Eighteen gametophytes includingL. japonica, L. ochotensisandL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria, but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species ofLaminaria. |
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| Keywords: | Laminaria gametophyte RAPD germplasm identification |
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