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DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTHIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS
作者姓名:刘建国  殷明炎  张京浦  刘伟  孟昭才
作者单位:R&D Center of Marine Biotechnology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China
基金项目:Projects3 95 0 0 1 1 43 9970 5 75 A/2 786 1and 95 65supportedbyNSFCIFS (InternationalFoundationforSciences),Sci TechCommissionofShandongProvincerespectively
摘    要:INTRODUCTIONOverthepastdecade ,Haematococcuspluvialishasbeenasubjectofnumerousinvestigationsbe causeitsconspicuousredcolorpointstoitspossibilityasahopefulregenerativebioresourceofastaxan thin .Extensivestudyofculturefactors (suchastemperature,illuminatio…


DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTHIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS
LIU Jian guo,YIN Ming yan,ZHANG Jing pu LIU Wei,MENG Zhao cai.DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTHIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS[J].Chinese Journal of Oceanology and Limnology,2002,20(4).
Authors:LIU Jian guo  YIN Ming yan  ZHANG Jing pu LIU Wei  MENG Zhao cai
Abstract:This study on dynamic changes of culture color, astaxanthin and chlorophylls, inorganic N including N-NO-3, N-NO-2 and N-NH+4 in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentrati on showed (1) ast/chl ratio was over 0.8 for brown and red algae, but was usually less than 0. 5 for green and yellow algae; (2) N-NO-3, in general, was unstable and decreased , except for a small unexpected increase in nitrate enriched treatment groups; (3) mea surable amounts of N-NO-2 and N-NH+4 were observed respectively with three cha nge modes although no external nitrite and ammonia were added into the culture; (4) a non-linear correlation between ast/chl ratio (or color) changes and th e levels of N-NO-3 , N-NO-2 , N-NH+4 in H. pluvialis culture; (5) up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown (or red) when N-NO-3, N-NO-2 and N-NH+ 4 fluctuated around 30, 5, 5 μmol/L respectively; (6) existence of three d ynamic modes of N-NO-3, N-NO-2 and N-NH+4 changes, obviously associated with initial external nitrate; (7) the key level of total inorganic N concentration regulatin g the above physiological changes during indoor cultivation was about 50 μmol/L ; and (8) 0.5-10 mmol/L of nitrate was theoretically conducive to cell growth in batch culture.
Keywords:Haematococcus pluvialis      nitrate    nitrite    ammonia    astaxanthin
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