Molecular identification and culture observation on <Emphasis Type="Italic">Acrochaete leptochaete</Emphasis> (Chaetophoraceae,Chlorophyta) from China |
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| Authors: | Yunyan?Deng Xiaorong?Tang Bingxin?Huang Linhong?Teng Email author" target="_blank">Lanping?DingEmail author |
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| Institution: | 1, 4, ** 1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China 3 Marine Genetics and Breeding Laboratory, Ocean University of China, Qingdao 266003, China 4 Shantou University, Shantou 515063, China |
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| Abstract: | Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae, Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain
the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth
characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self-replicating generations. Zoospore
germination was bipolar. A temperature range from 13–21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species’ general biology
and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations
are applied. |
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| Keywords: | Acrochaete leptochaete asexual reproduction culture observation growth characteristics molecular identification |
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